Interactive impacts of microplastics and arsenic on agricultural soil and plant traits

Abstract

The ability of microplastics (MPs) to interact with environmental pollutants is currently of great concern due to the increasing use of plastic. Agricultural soils are sinks for multipollutants and the safety of biodegradable MPs in field conditions is questioned. However, still few studies have investigated the interactive effects between MPs and metals on the soil-plant system with agricultural soil and testing crops for human consumption. In this work, we tested the effect on soil and plant parameters of two common MPs, non-degradable plastic low-density polyethylene and biodegradable polymer polylactic acid at two different sizes (<250 μm and 250-300 μm) in association with arsenic (As). Lettuce (Lactuca sativa L.) was used as a model plant in a small-scale experiment lasting 60 days. Microplastics and As explained 12 % and 47 % of total variance, respectively, while their interaction explained 21 %, suggesting a higher toxic impact of As than MPs. Plant growth was promoted by MPs alone, especially when biodegradable MPs were added (+22 %). However, MPs did not affect nutrient concentrations in roots and leaves. The effect of MPs on enzyme activities was variable depending on the time of exposure (with larger effects immediately after exposure), the type and size of the MPs. On the contrary, the co-application of MP and As, although it did not change the amount of bioavailable As in soil in the short and medium term, it resulted in a significant decrease in lettuce biomass (-19 %) and root nutrient concentrations, especially when polylactic acid was applied. Generally, MPs in association with As determined the plant-soil toxicity. This work provides insights into the risk of copollution of MPs and As in agricultural soil and its phytotoxic effect for agricultural crops. However, the mechanisms of the joint effect of MP and As on plant toxicity need further investigation, especially under field conditions and in long-term experiments.

Keywords: Low-density polyethylene; Plant toxicity; Plant-soil system; Polylactic acid; Soil contamination; Soil enzyme activity.