Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue

Jemma Gasperoni; Sebastian Dworkin

doi:10.1007/978-1-0716-3585-8_13

Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue

Methods Mol Biol. 2024:2746:155-163. doi: 10.1007/978-1-0716-3585-8_13.

Authors

Jemma Gasperoni¹, Sebastian Dworkin²

Affiliations

¹ Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC, Australia.
² Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC, Australia. s.dworkin@latrobe.edu.au.

PMID: 38070088
DOI: 10.1007/978-1-0716-3585-8_13

Abstract

Cryopreservation and immunohistochemistry offer a comprehensive, robust, and simple methodology to investigate neural patterning and cellular function. Rapid freezing of the whole brain allows excellent preservation of neural ultrastructure and tissue architecture without destroying sensitive protein epitopes that are often compromised following standard paraffin embedding histological techniques. Here, we present a rapid and simple protocol for employing cryosectioning and subsequent immunohistochemistry in the study of adult murine brain neural tissue.

Keywords: Brain; Cryopreservation; Cryosectioning; Immunohistochemistry; Mouse.

MeSH terms

Animals
Brain
Cryopreservation* / methods
Cryoultramicrotomy*
Freezing
Immunohistochemistry
Mice