Neural Stem/Progenitor Cell (NSPC) Extraction and Culture

Jemma Gasperoni; Sebastian Dworkin

doi:10.1007/978-1-0716-3585-8_9

Neural Stem/Progenitor Cell (NSPC) Extraction and Culture

Methods Mol Biol. 2024:2746:109-120. doi: 10.1007/978-1-0716-3585-8_9.

Authors

Jemma Gasperoni¹, Sebastian Dworkin²

Affiliations

¹ Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC, Australia.
² Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC, Australia. s.dworkin@latrobe.edu.au.

PMID: 38070084
DOI: 10.1007/978-1-0716-3585-8_9

Abstract

Neural stem-progenitor cells (NSPCs) are multipotent, self-renewing cells that generate radial glial cells (RGC). RGCs then give rise to neurons and glia during neural development. Here, we describe the process of NSPC isolation and culturing to form clonal aggregates termed neurospheres. There are multiple assays outlined in this chapter that allow us to quantify differences in proliferation, self-renewal potential, and differentiation of these cells.

Keywords: Brain; Cell culture; Differentiation; Mouse; Neurons; Neurosphere; Stem cells.

MeSH terms

Cell Differentiation / physiology
Cell Proliferation
Cells, Cultured
Multipotent Stem Cells
Neural Stem Cells*
Neuroglia
Neurons