High-Throughput Multiplex Immunohistochemistry of Glioma Organoids

Marija Dinevska; Samuel S Widodo; Theo Mantamadiotis

doi:10.1007/978-1-0716-3585-8_4

High-Throughput Multiplex Immunohistochemistry of Glioma Organoids

Methods Mol Biol. 2024:2746:57-65. doi: 10.1007/978-1-0716-3585-8_4.

Authors

Marija Dinevska¹, Samuel S Widodo², Theo Mantamadiotis^{2

3

4}

Affiliations

¹ Department of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia. mdinevska@student.unimelb.edu.au.
² Department of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia.
³ Department of Microbiology and Immunology, The University of Melbourne, Melbourne, VIC, Australia.
⁴ Centre for Stem Cell Systems, The University of Melbourne, Parkville, VIC, Australia.

PMID: 38070079
DOI: 10.1007/978-1-0716-3585-8_4

Abstract

The invasive capacity and progression of glioblastoma cells and neoplastic cells in other are dependent on interactions with the surrounding tumor microenvironment. In particular, cancer cells form a reciprocal relationship with noncellular dysregulated extracellular matrix in the tumors. Here, we describe a protocol that can be used to model the functional relationship between tumor cells and extracellular matrix. We demonstrate how 3D organoids, including glioma tumor organoids, can be processed, embedded, and sectioned in a high-throughput setup that enables investigation of the organoids by histopathological methods, multiplex immunohistochemistry, and spatial analysis within the same section.

Keywords: Glioblastoma; Glioma; Multiplex immunohistochemistry; Organoid; Spheroid microarray.

MeSH terms

Glioblastoma* / pathology
Humans
Immunohistochemistry
Organoids / pathology
Tumor Microenvironment