(Very) Small Stem-like Cells in Human Cell Cultures

Jan Jakub Lica; Bhaskar Pradhan

doi:10.3390/cancers15235520

(Very) Small Stem-like Cells in Human Cell Cultures

Cancers (Basel). 2023 Nov 22;15(23):5520. doi: 10.3390/cancers15235520.

Authors

Jan Jakub Lica^{1

2}, Bhaskar Pradhan³

Affiliations

¹ Department of Regenerative Medicine, Faculty of Medicine, Medical University of Warsaw, 02-097 Warsaw, Poland.
² Department of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, 80-308 Gdansk, Poland.
³ Department of Biochemistry, Faculty of Pharmacy, Medical University of Warsaw, 02-097 Warsaw, Poland.

Abstract

Very Small Embryonic-like Stem Cells (VSELSCs) and Very Small Cancer Stem Cells (VSCSCs) are fields of intensive research. Although the presence in vitro of VSELSC and VSCSC cellular stage analogs appear probable, it has yet to be published. Utilizing established human cell cultures with varying populations of primitive cells, stained with CD markers specific to primitive stages, in addition to a fluorescent DNA dye, and following histochemical processing, we have developed a cytological method for detecting Very Small Leukemic Stem-like Cells (VSLSLCs), Very Small Cancer Stem-like Cells (VSCSLCs), and VSELSCs. This detection provides an opportunity to advance research in these areas.

Keywords: cancer stem-like cells; cell cultures: A549, HEK293, HL-60, Jurkat, KG1a, K562, and Raji; cluster of differentiation: CD34, CD45, CD56, and CD2; leukemic stem-like cells; very small cancer stem-like cells; very small embryonic-like stem cells; very small leukemic stem-like cells.

Abstract

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