Reverse Genetics Systems for Filoviruses

Abstract

Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable. Here, we describe the generation of recombinant filoviruses from cDNA.

Keywords: Ebola virus; Filoviruses; Full-length clone system; Infectious clone; Marburg virus; Recombinant virus; Reverse genetics.