Ultrasensitive quantification of HIV-1 cell-to-cell transmission in primary human CD4+ T cells measures viral sensitivity to broadly neutralizing antibodies

Dmitriy Mazurov; Alon Herschhorn

doi:10.1128/mbio.02428-23

Ultrasensitive quantification of HIV-1 cell-to-cell transmission in primary human CD4⁺ T cells measures viral sensitivity to broadly neutralizing antibodies

mBio. 2024 Jan 16;15(1):e0242823. doi: 10.1128/mbio.02428-23. Epub 2023 Dec 8.

Authors

Dmitriy Mazurov¹, Alon Herschhorn^{1

2

3

4

5

6}

Affiliations

¹ Division of Infectious Diseases and International Medicine, Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA.
² Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA.
³ Institute for Engineering in Medicine, Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
⁴ Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, Minnesota, USA.
⁵ The College of Veterinary Medicine Graduate Program, University of Minnesota, Minneapolis, Minnesota, USA.
⁶ Molecular Pharmacology and Therapeutics Graduate Program, University of Minnesota, Minneapolis, Minnesota, USA.

Abstract

HIV-1 can efficiently transmit from one cell to another but accurate quantification of this mode of transmission is still challenging. Here, we developed an ultrasensitive assay to measure HIV-1 transmission between cells and to evaluate HIV-1 escape from broadly neutralizing antibodies in primary human T cells. This assay will contribute to understanding the fundamental mechanisms of HIV-1 cell-to-cell transmission, allow evaluation of pre-existing or acquired HIV-1 resistance in clinical trials, and can be adapted to study the biology of other retroviruses.

Keywords: HIV-1; broadly neutralizing antibodies; cell-cell transmission.

MeSH terms

Antibodies, Neutralizing
Broadly Neutralizing Antibodies
CD4-Positive T-Lymphocytes
HIV Antibodies
HIV Infections*
HIV Seropositivity*
HIV-1*
Humans
T-Lymphocytes

Substances

Broadly Neutralizing Antibodies
HIV Antibodies
Antibodies, Neutralizing

Abstract

MeSH terms

Substances

Grants and funding