Novel electrochemiluminescence aptasensor based on AuNPs-ABEI encapsulated TiO2 nanorod for the detection of acetamiprid residues in vegetables

Jiashuai Sun; Wenzheng Liu; Zhenying He; Baoxin Li; Haowei Dong; Mengyue Liu; Jingcheng Huang; Peisen Li; Donghan Li; Yingchao Xu; Shancang Zhao; Yemin Guo; Xia Sun

doi:10.1016/j.talanta.2023.125471

Novel electrochemiluminescence aptasensor based on AuNPs-ABEI encapsulated TiO₂ nanorod for the detection of acetamiprid residues in vegetables

Talanta. 2024 Mar 1:269:125471. doi: 10.1016/j.talanta.2023.125471. Epub 2023 Dec 6.

Authors

Jiashuai Sun¹, Wenzheng Liu¹, Zhenying He¹, Baoxin Li¹, Haowei Dong¹, Mengyue Liu¹, Jingcheng Huang¹, Peisen Li¹, Donghan Li¹, Yingchao Xu², Shancang Zhao³, Yemin Guo⁴, Xia Sun⁵

Affiliations

¹ College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
² College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
³ College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
⁴ College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: gym@sdut.edu.cn.
⁵ College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. Electronic address: sunxia2151@sina.com.

PMID: 38061203
DOI: 10.1016/j.talanta.2023.125471

Abstract

Gold nanoparticles (AuNPs)@N-(4-aminobutyl)-N-ethylisoluminol (ABEI)@Titanium dioxide nanorods (TiO₂NRs) were used as sensing materials to produce a unique encapsulated nanostructure aptasensor for the detection of acetamiprid residues in this work. ABEI, an analog of luminol, was extensively used as an electrochemiluminescence (ECL) reagent. The ECL mechanism of ABEI- hydrogen peroxide (H₂O₂) system had connections to a number of oxygen-centered free radicals. TiO₂NRs improved ECL response with high electron transfer and a specific surface area. AuNPs were easy to biolabel and could catalyze H₂O₂ to enhance ECL signal. AuNPs were wrapped around TiO₂NRs by utilizing the reduction property of ABEI to form wrapped modified nanomaterials. The sulfhydryl-modified aptamer bound to the nanomaterial by forming gold-sulfur (Au-S) bonds. The aptamer selectively bound to its target with the addition of acetamiprid, which caused a considerable decrease in ECL intensity and enabled quantitative detection of acetamiprid. The aptasensor showed good stability, repeatability and specificity with a broad detection range (1×10^-2-1×10³ nM) and a lower limit of detection (3 pM) for acetamiprid residues in vegetables. Overall, this aptasensor presents a simple and highly sensitive method for ECL detecting acetamiprid, with potential applications in vegetable safety monitoring.

Keywords: Acetamiprid; Aptasensor; Electrochemiluminescence; Functionalized nanomaterials; Vegetables.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Gold / chemistry
Hydrogen Peroxide / chemistry
Limit of Detection
Luminescent Measurements / methods
Luminol / chemistry
Metal Nanoparticles* / chemistry
Nanotubes*
Vegetables

Substances

6-(N-(4-aminobutyl)-N-ethyl)amino-2,3-dihydrophthalazine-1,4-dione
Gold
acetamiprid
Hydrogen Peroxide
Luminol
Aptamers, Nucleotide