A potential mechanism of miana (Coleus scutellariodes) and quercetin via NF-κB in Salmonella typhi infection

Ade Rifka Junita; Firdaus Hamid; Budu Budu; Rosdiana Natzir; Yusmina Hala; Gemini Alam; Rosana Agus; Burhanuddin Bahar; Ahmad Syukri; Muhammad Reza Primaguna; Ressy Dwiyanti; Andini Febrianti; Muhammad Sabir; Azhar Azhar; Mochammad Hatta

doi:10.1016/j.heliyon.2023.e22327

A potential mechanism of miana (Coleus scutellariodes) and quercetin via NF-κB in Salmonella typhi infection

Heliyon. 2023 Nov 14;9(11):e22327. doi: 10.1016/j.heliyon.2023.e22327. eCollection 2023 Nov.

Authors

Ade Rifka Junita^{1

2}, Firdaus Hamid³, Budu Budu⁴, Rosdiana Natzir⁵, Yusmina Hala⁶, Gemini Alam⁷, Rosana Agus⁷, Burhanuddin Bahar⁸, Ahmad Syukri^{1

2}, Muhammad Reza Primaguna⁹, Ressy Dwiyanti^{10

11}, Andini Febrianti¹¹, Muhammad Sabir¹⁰, Azhar Azhar¹², Mochammad Hatta²

Affiliations

¹ Postgraduate School, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
² Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
³ Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
⁴ Department of Ophthalmology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
⁵ Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
⁶ Department of Biology, Faculty of Sciences, State University of Makassar, Makassar, Indonesia.
⁷ Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia.
⁸ Department of Biostatistic, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
⁹ Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
¹⁰ Department of Medical Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia.
¹¹ Department of Forensic and Medicolegal, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
¹² Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.

Abstract

Purpose: To prove the effect of Miana (M), Quercetin (Q), and the combination as an anti-inflammatory agent and Cefixime (C) as an antibiotic in Balb/c mice infected with Salmonella enterica serovar Typhi (S. Typhi) and related to the dynamics of NF-κB mRNA expression and NF-κB protein levels.

Methods: A cohort study on male Balb/c mice with subjects consisted of 8 groups with 5 each group by administration of M, Q, M + Q, M + C, Q + C, M + Q + C, C only and sterile distilled water group as negative control. The statistical significance of the difference group was defined as P values less than 0.05.

Results: Decreased mRNA expression of NF-κB, NF-κB protein levels, and bacterial load after administration of M + C, Q + C, or M + Q + C showed significant differences when compared to the negative control. The decline in NF-κB was stronger when M + Q + C was given compared to M, Q, M + Q, or C only.

Conclusion: The effects of NF-κB suppression appear to be the same between the administration of M, Q and the M + Q when C added. However, the suppression of NF-κB was not significant without adding C.

Keywords: Anti-inflammation; Antibacterial; Miana (Coleus scutellariodes); Nuclear factor-kappa B (NF-κB); Quercetin; Salmonella enterica serovar typhi (S. typhi).