Abstract
Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the offtarget problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized. [BMB Reports 2024; 57(1): 12-18].
MeSH terms
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CRISPR-Cas Systems* / genetics
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Clustered Regularly Interspaced Short Palindromic Repeats / genetics
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Gene Editing* / methods
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Genome / genetics
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Technology
Grants and funding
ACKNOWLEDGEMENTS This research was supported by grants from the National Research Foundation funded by the Korean Ministry of Education, Science and Technology (NRF-2022R1A2C4001609) and the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Health & Welfare) (22A0203L1). This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2023.