Cytotoxicity evaluation of phytochemicals from stingless bee (Tetragonula biroi) propolis

Rico Ramadhan; Syafrizal; Irawan Wijaya Kusuma; Swandari Paramita; Yhiya Amen; Yong-Ung Kim; Widya Fatriasari; Kuniyoshi Shimizu; Enos Tangke Arung

doi:10.1016/j.toxicon.2023.107352

Cytotoxicity evaluation of phytochemicals from stingless bee (Tetragonula biroi) propolis

Toxicon. 2024 Jan:237:107352. doi: 10.1016/j.toxicon.2023.107352. Epub 2023 Dec 3.

Authors

Rico Ramadhan¹, Syafrizal², Irawan Wijaya Kusuma³, Swandari Paramita⁴, Yhiya Amen⁵, Yong-Ung Kim⁶, Widya Fatriasari⁷, Kuniyoshi Shimizu⁸, Enos Tangke Arung⁹

Affiliations

¹ Division of Exploration and Synthesis of Bioactive Compounds (ESBC), CoE-University Research Center for Bio-Molecule Engineering (BIOME), Universitas Airlangga, Surabaya, 60115, Indonesia; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
² Laboratory of Plant Systematic and Anatomy, Mathematics and Science Faculty, Mulawarman University, Samarinda, Indonesia.
³ Laboratory of Forest Product Chemistry, Faculty of Forestry, Mulawarman University, Samarinda, Indonesia; Research Center for Medicine and Cosmetic from Tropical Rainforest Resources, Mulawarman University, Samarinda, Indonesia.
⁴ Research Center for Medicine and Cosmetic from Tropical Rainforest Resources, Mulawarman University, Samarinda, Indonesia; Faculty of Medicine, Mulawarman University, Samarinda, Indonesia.
⁵ Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
⁶ Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan, South Korea.
⁷ Research Center for Biomass and Bioproducts, National Research and Innovation Agency, Bogor, 16911, Indonesia; Research Collaboration Center for Biomass-Based Nano Cosmetic, in Collaboration Mulawarman University and BRIN, Samarinda, East Kalimantan, 75119, Indonesia.
⁸ Department of Agro-Environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan; Kyushu University Institute for Asian and Oceanian Studies, Fukuoka, Japan. Electronic address: shimizu@agr.kyushu-u.ac.jp.
⁹ Laboratory of Forest Product Chemistry, Faculty of Forestry, Mulawarman University, Samarinda, Indonesia; Research Center for Medicine and Cosmetic from Tropical Rainforest Resources, Mulawarman University, Samarinda, Indonesia; Research Collaboration Center for Biomass-Based Nano Cosmetic, in Collaboration Mulawarman University and BRIN, Samarinda, East Kalimantan, 75119, Indonesia. Electronic address: tangkearung@yahoo.com.

PMID: 38052333
DOI: 10.1016/j.toxicon.2023.107352

Abstract

Three prenylated flavonoids (1-3) were isolated from Tetragonula biroi propolis. The structures of the isolated compounds were characterized by NMR, IR, and UV spectroscopic and mass spectrometric analyses. The cytotoxicity activity of the crude extracts, fractions and the isolated compounds were established against four cell lines such as Caco-2, HeLa, MCF-7, and OVK-18. Among the tested compounds, compound 1 showed cytotoxicity activity against MCF-7 cell lines, whereas compound 2 showed good activity against Caco-2 and OVK-18 cell lines with IC₅₀ values of 14.73 and 14.44, respectively. Moreover, compound 3 exhibited strong activity against OVK-18 cell lines. These findings contribute to the phytochemical understanding of the T. biroi propolis, and their cytotoxicity effects for future pharmaceutical purposes.

Keywords: Anti-cancer; Cytotoxicity; Propolis; Tetragonula biroi.

MeSH terms

Animals
Bees
Caco-2 Cells
Complex Mixtures
Humans
Phytochemicals / toxicity
Propolis* / chemistry
Propolis* / pharmacology

Substances

Propolis
Complex Mixtures
Phytochemicals