Morphology and molecular phylogeny of Neolentinus in northern China

Lei Yue; Yong-Lan Tuo; Zheng-Xiang Qi; Jia-Jun Hu; Ya-Jie Liu; Xue-Fei Li; Ming-Hao Liu; Bo Zhang; Shu-Yan Liu; Yu Li

doi:10.7717/peerj.16470

Morphology and molecular phylogeny of Neolentinus in northern China

PeerJ. 2023 Nov 30:11:e16470. doi: 10.7717/peerj.16470. eCollection 2023.

Authors

Lei Yue^#^{1

2}, Yong-Lan Tuo^#^{1

2}, Zheng-Xiang Qi^{1

2}, Jia-Jun Hu^{1

3}, Ya-Jie Liu^{1

2}, Xue-Fei Li^{1

2}, Ming-Hao Liu^{1

2}, Bo Zhang^{1

2}, Shu-Yan Liu^{1

2}, Yu Li^{1

2

4}

Affiliations

¹ Engineering Research Centre of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun City, Jilin Province, China.
² College of Plant Protection, Jilin Agricultural University, Changchun City, Jilin Province, China.
³ School of Life Science, Zhejiang Normal University, Jinhua City, Zhejiang Province, China.
⁴ Mycological Valley Innovation Institute (Hefei), Hefei City, Anhui Province, China.

^# Contributed equally.

Abstract

Neolentinus is a significant genus, belonging to Gloeophyllaceae, with important economic and ecological values, which are parasites on decaying wood of broad-leaf or coniferous trees, and will cause brown rot. However, the taxonomic study is lagging behind to other groups of macrofungi, especially in China. In view of this, we conducted morphological and molecular phylogenetic studies on this genus. We have discovered new types of cheilocystidia and with extremely long lamellae in Neolentinus, and, thus proposed it as a new species-Neolentinus longifolius. At the same time, we clarified the distribution of Neolentinus cyathiformis in China and provided a detailed description. Moreover, we also described two common species, viz. Neolentinus lepideus and Neolentinus adhaerens. All the species are described based on the Chinese collections. The key to the reported species of Neolentinus from China is provided. And the phylogeny of Neolentinus from China is reconstructed based on DNA sequences of multiple loci including the internal transcribed spacer (ITS) regions, the large subunit nuclear ribosomal RNA gene (nLSU), and the translation elongation factor 1-α gene (tef-1α). In addition, full morphological descriptions, illustrations, color photographs, taxonomic notes, and all the available sequences of Neolentinus species are provided.

Keywords: Cheilocystidia; Neolentinus longifolius; New character; New species.

MeSH terms

China
Phylogeny
Wood*

Grants and funding

This research was funded by the Jilin Province Science and Technology Development Plan Project (No. 20230202112NC), the Wildlife and Plant Resources Investigation Project in Hani National Nature Reserve, Jilin Province (HX-2023003), the Research on the Creation of Excellent Edible Mushroom Resources and High Quality & Efficient Ecological Cultivation Technology in Jiangxi Province (20212BBF61002), and the Scientific and Technological Tackling Plan for the Key Fields of Xinjiang Production and Construction Corps (No. 2021AB004). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.