Chemosensitivity analysis of tiger nuts (Cyperus esculentus L.) using ethyl methanesulfonate (EMS) and colchicine mutagens

Patrick Twumasi; Pual Agu Asare; Emmanuel Afutu; Godwin Amenorpe; Sylvester N T T Addy

doi:10.1016/j.heliyon.2023.e22619

Chemosensitivity analysis of tiger nuts (Cyperus esculentus L.) using ethyl methanesulfonate (EMS) and colchicine mutagens

Heliyon. 2023 Nov 19;9(12):e22619. doi: 10.1016/j.heliyon.2023.e22619. eCollection 2023 Dec.

Authors

Patrick Twumasi¹, Pual Agu Asare¹, Emmanuel Afutu¹, Godwin Amenorpe², Sylvester N T T Addy³

Affiliations

¹ Department of Crop Science, University of Cape Coast (UCC), Ghana.
² Biotechnology and Nuclear Agriculture Research Institute (BNARI), Ghana Atomic Energy Commission (GAEC), Ghana.
³ Center for Scientific and Industrial Research - Crop Research Institute (CSIR-CRI), Fumesua, Ghana.

Abstract

Four hundred tubers of four genotypes, two brown and two black tiger nuts were subjected to Ethyl Methanesulfonate (EMS) and Colchicine treatments at concentrations of 0 %, 0.1 %, 0.25 %, 0.5 % and 1.0 % for 24 h. Each genotype had twenty tubers treated with each of the five different concentrations and were planted using Complete Randomized Design (CRD) in a greenhouse. Quantitative data was collected and LD₅₀ and RD₅₀ were analysed using Excell 2016 and Genstat 11.2. A general decreasing trend in percentage germination and plant height was observed with increasing concentrations of mutagens applied. An EMS treatment had LD₅₀ and RD₅₀ values of 0.97 % and 1.49 % for black and 0.63 % and 1.63 % for brown genotypes. Similarly, the percentage colchicine treatment had LD₅₀ and RD₅₀ values of 1.65 % and 19.51 % concentrations for black and 0.91 % and 1.71 % concentrations for brown genotypes.

Keywords: Colchicine; Concentration levels; EMS; Genotype; LD50; Mutagens; Plant architecture; RD50.