Morphometric features, seminal profile and diluters effect on post-thaw semen quality of Munshiganj cattle in Bangladesh

Dipa Das; Mohammad Mahbubul; Md Younus Ali; Md Samim Kobir; Md Ahsanul Kabir; Sheikh Mohammad Jahangir Hossain; Auvijit Saha Apu

doi:10.1016/j.heliyon.2023.e21967

Morphometric features, seminal profile and diluters effect on post-thaw semen quality of Munshiganj cattle in Bangladesh

Heliyon. 2023 Nov 7;9(12):e21967. doi: 10.1016/j.heliyon.2023.e21967. eCollection 2023 Dec.

Authors

Dipa Das^{1

2}, Mohammad Mahbubul³, Md Younus Ali¹, Md Samim Kobir¹, Md Ahsanul Kabir², Sheikh Mohammad Jahangir Hossain², Auvijit Saha Apu¹

Affiliations

¹ Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
² Bangladesh Livestock Research Institute, Savar, Dhaka, 1341, Bangladesh.
³ Haor and Char Development Institute, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.

Abstract

The study investigates morphometric features, seminal profile and post-thaw semen quality of Munshiganj cattle. Morphometric features were measured using measuring tape from 20 Munshiganj cattle while coat color was measured by observing in naked eye. Fresh and post thaw semen quality parameters were analyzed using Computer Assisted semen analyzer (CASA). Coat color of Munshiganj male cattle were creamy white to dull pinkish and female were white to creamy. The mean body weight, body length, hearth girth, height at wither, head length, head width, ear length, ear width, fore leg length, hind leg length, tail length, tail doc circumference, horn length, horn diameter and mouth circumference were 362.80 kg, 137.31 cm, 160.66 cm, 135.21 cm, 50.97 cm, 20.58 cm, 19.75 cm, 9.88 cm, 73.02 cm, 74.84 cm, 106.10 cm, 20.75 cm, 13.60 cm, 16.12 cm and 43.00 cm, respectively. There was significant difference (p < 0.05) between male and female in terms of body weight (418.00 vs 307.60 kg), heart girth (173.74 vs 147.57 cm), head width (22.50 vs 18.67 cm), horn diameter (18.58 vs 13.66 cm) and mouth circumference (46.60 vs 39.40 cm). Average scrotal length was 16.76 cm while scrotal circumference was 32.70 cm. Age had significant effect (p < 0.05) on morphometric characteristics of Munshiganj male and female cattle. On the other hand, season had no significant effect on semen quality. Mean semen volume, sperm concentration, total motile, progressive, static, slow and live spermatozoa were 5.83 ± 0.88 ml, 1510.27 ± 844.07 million/ml, 91.9 ± 2.17 %, 63.80 ± 12.53 %, 8.10 ± 2.17 %, 0.10 ± 0.10 %, 91.38 ± 0.25 %, respectively. On the other hand, sperm head length and width, sperm tail length of Munshiganj cattle were 10.39 ± 0.16 μm, 4.26 ± 0.07 μm, 21.5 ± 0.52 μm, respectively. Individual breeding bull had a significant (p < 0.05) effect on post-thawed motile sperm percentage. Four different diluters (Triladyl, Steridyl, Tris-egg yolk-Citrate and Andromed) were used to compare the effects of diluter on post-thaw semen quality of Munshiganj cattle and found that diluter had no significant effect (p > 0.05) on post thaw semen quality except slow motility and proximal droplet percentages. Munshiganj cattle had a distinctive phenotypic feature with standard quality semen and had no effect of egg yolk free and egg yolk based diluters on post thaw semen quality.

Keywords: Conservation; Cryopreservation; Genetic resource; Morphometric.