Polydopamine@ZIFs with enhanced electrochemiluminescence quenching performance for mycotoxin detection

Qing Yang; Jianhua Xiong; Luying Duan; Sida Chen; Zhongji Peng; Xiaoning Liao; Zhenqiang Ning; Dan Wang

doi:10.1016/j.foodchem.2023.138058

Polydopamine@ZIFs with enhanced electrochemiluminescence quenching performance for mycotoxin detection

Food Chem. 2024 May 1:439:138058. doi: 10.1016/j.foodchem.2023.138058. Epub 2023 Nov 30.

Authors

Qing Yang¹, Jianhua Xiong¹, Luying Duan¹, Sida Chen², Zhongji Peng¹, Xiaoning Liao³, Zhenqiang Ning⁴, Dan Wang⁵

Affiliations

¹ Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
² Institute of Food Testing, Jiangxi General Institute of Testing and Certification, Nanchang 330052, China.
³ Research Center of Mycotoxins, Jiangxi Agricultural University, Nanchang 330045, China.
⁴ Jiangxi Provincial Key Laboratory of Laboratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.
⁵ Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address: WDAN1983@126.com.

PMID: 38043277
DOI: 10.1016/j.foodchem.2023.138058

Abstract

Quench-type electrochemiluminescence (ECL) immunosensors are appealing for detecting small molecule contaminants in signal-on mode, for which efficient ECL quenchers are highly desirable. Here, the classical quencher of polydopamine (PDA) was transformed into a unique structure by introducing zeolite imidazole frameworks (ZIFs). Besides the inherent energy transfer quenching effect on ECL, the resulting PDA@ZIFs exhibits a high scavenging property against electrogenerated coreactant-radicals and inhibits the formation of excited luminophore. A quench-type ECL immunosensor for ochratoxin A (OTA) was developed using the PDA@ZIFs as a quencher and the g-C₃N₄ as a luminophore. The immunosensor showed a good response towards the OTA with a linear range of 10.0 fg/mL-1.0 ng/mL and a detection limit of 4.8 fg/mL. Acceptable recoveries of 85.7 to 109.2 % were achieved for the detection of OTA in spiked foods. This work offers valuable insight for improving the performance of quench-typed ECL biosensors.

Keywords: Coreactant Radicals; Immunoassay; Quench-Type; Small Molecule Contaminants; Zeolitic Imidazolate Frameworks.

MeSH terms

Biosensing Techniques* / methods
Electrochemical Techniques / methods
Imidazoles
Immunoassay / methods
Limit of Detection
Luminescent Measurements / methods
Metal Nanoparticles* / chemistry
Mycotoxins*
Zeolites*

Substances

polydopamine
Mycotoxins
Zeolites
Imidazoles