Optimization of radioprotective dose of Juglans nigra leaf extract using response surface methodology

Abstract

J. nigra leaf contains mixture of various pharmacologically active compounds and it is assumed that they may have the potential radioprotective effect. The purpose of this work was to predict radioprotective doses by correlating changes in organ distribution of radiopharmaceuticals with extract dose levels and rat body weight using response surface methodology (RSM) based on a second-order polynomial equation. The extract was administered daily by oral gavage to rats at dose of 6.9, 10.3, or 13.7 mg kg^-1 body weight (bw) day^-1 for 10 days. On the eleventh day, 0.1 ml of the one radiopharmaceutical (Na^99mTcO₄, ^99mTc-dimercaptosuccinic acid and ^99mTc-tin colloid) was injected into the tail vein. The statistical parameters: the coefficient of determination (0.81-0.95), the coefficient of variation (3.05-11.1), the adequate precision (8.82-19.12) and the mean relative percentage deviation (± 2.3-8.2) were indicated the precision and reliability of RSM. Using RSM, the predicted daily dose of leaf extract ranging from 11.19 to 11.99 mg kg^-1 bw may be considered as an optimal daily radiopotective dose for protection of organs from radiation in cases of planned radiation exposures.

Keywords: (99m)Tc-dimercaptosuccinic acid; (99m)Tc-tin colloid; J. nigra leaf; Na(99m)TcO(4); Optimization; Response surface methodology.