Potential Association of The Pathogenic Kruppel-like Factor 14 (KLF14) and Adiponectin (ADIPOQ) SNVs with Susceptibility to T2DM

Imadeldin Elfaki; Rashid Mir; Faris Tayeb; Adel Ibrahim Ahmad Al-Alawy; Jameel Barnawi; Pradeep K Dabla; Mamdoh S Moawadh

doi:10.2174/0118715303258744231117064253

Potential Association of The Pathogenic Kruppel-like Factor 14 (KLF14) and Adiponectin (ADIPOQ) SNVs with Susceptibility to T2DM

Endocr Metab Immune Disord Drug Targets. 2023 Nov 28. doi: 10.2174/0118715303258744231117064253. Online ahead of print.

Authors

Imadeldin Elfaki¹, Rashid Mir², Faris Tayeb², Adel Ibrahim Ahmad Al-Alawy¹, Jameel Barnawi², Pradeep K Dabla³, Mamdoh S Moawadh²

Affiliations

¹ Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 47713, Saudi Arabia.
² Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 47713, Saudi Arabia.
³ Department of Biochemistry, Govind Ballabh Pant Institute of Postgraduate Medical Education & Research (GIPMER), Associated to Maulana Azad Medical College, Delhi 110002, India Department of Biochemistry, Govind Ballabh Pant Institute of Postgraduate Medical Education & Research (GIPMER), Associated to Maulana Azad Medical College, Delhi 110002, India dahli India.

PMID: 38031795
DOI: 10.2174/0118715303258744231117064253

Abstract

Aim: To evaluate the associations of the pathogenic variants in Kruppel-like Factor 14 (KLF 14) and Adiponectin (ADIPOQ) with susceptibility to type 2 diabetes mellitus (T2DM).

Background: Type 2 diabetes mellitus (T2DM) is a pandemic metabolic disease characterized by increased blood sugar and caused by resistance to insulin in peripheral tissues and damage to pancreatic beta cells. Kruppel-like Factor 14 (KLF-14) is proposed to be a regulator of metabolic diseases, such as diabetes mellitus (DM) and obesity. Adiponectin (ADIPOQ) is an adipocytokine produced by the adipocytes and other tissues and was reported to be involved in T2DM.

Objectives: To study the possible association of the KLF-14 rs972283 and ADIPOQ-rs266729 with the risk of T2DM in the Saudi population.

Methods: We have evaluated the association of KLF-14 rs972283 C>T and ADIPOQ-rs266729 C>G SNV with the risk to T2D in the Saudi population using the Amplification Refractory Mutation System PCR (ARMS-PCR), and blood biochemistry analysis. For the KLF-14 rs972283 C>T SNV we included 115 cases and 116 healthy controls, and ADIPOQ-rs266729 C>G SNV, 103 cases and 104 healthy controls were included.

Results: Results indicated that the KLF-14 rs972283 GA genotype and A allele were associated with T2D risk with OR=2.14, p-value= 0.014 and OR=1.99, p-value=0.0003, respectively. Results also ADIPOQ-rs266729 CG genotype and C allele were associated with an elevated T2D risk with an OR=2.53, p=0.003 and OR=1.66, p-value =0.012, respectively.

Conclusion: We conclude that SNVs in KLF-14 and ADIPOQ are potential loci for T2D risk. Future large-scale studies to verify these findings are recommended. These results need further verifications in protein functional and large-scale case control studies before being introduced for genetic testing.

Keywords: Adiponectin ADIPOQ-rs266729; Amplification Refractory Mutation System PCR (ARMS-PCR); KLF-14 rs972283 SNV; Single nucleotide variations (SNVs); Type 2 diabetes.