Early revascularization activates quiescent dental pulp stem cells following tooth replantation in mice

Hiroto Sano; Kuniko Nakakura-Ohshima; Angela Quispe-Salcedo; Yasuo Okada; Takuichi Sato; Hayato Ohshima

doi:10.1016/j.reth.2023.10.004

Early revascularization activates quiescent dental pulp stem cells following tooth replantation in mice

Regen Ther. 2023 Nov 6:24:582-591. doi: 10.1016/j.reth.2023.10.004. eCollection 2023 Dec.

Authors

Hiroto Sano¹, Kuniko Nakakura-Ohshima², Angela Quispe-Salcedo³, Yasuo Okada¹, Takuichi Sato⁴, Hayato Ohshima³

Affiliations

¹ Department of Pathology, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan.
² Division of Pediatric Dentistry, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
³ Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
⁴ Division of Clinical Chemistry, Department of Medical Technology, Niigata University Graduate School of Health Sciences, Niigata, Japan.

Abstract

Introduction: The intentional perforation of the pulp chamber floor before tooth replantation promotes pulpal healing by facilitating the revascularization of the pulp cavity. This study aimed to elucidate the effects of this method on the dynamics of quiescent dental pulp stem cells (DPSCs).

Methods: The right and left maxillary first molars of Crlj:CD1 mice and TetOP-histone 2B (H2B)-green fluorescent protein (GFP) mice were extracted. The left molars were immediately replanted as the control group (CG), whereas the pulp chamber floor of the right molars were perforated before the tooth was replanted as the experimental group (EG). Immunohistochemistry for Nestin and GFP, and quantitative RT-PCR for Nestin, Opn, CD11c, and Oct3/4 mRNA were performed.

Results: The rate of Nestin-positive perimeter along the pulp-dentin border in the EG tended to be higher than that of the CG at days 5 and 7 and was significantly increased between days 3 and 7. The rate of GFP-positive cells in the EG was significantly higher than that of the CG at days 5 and/or 7 in the mesial and middle coronal pulp. CD11c mRNA in the EG at day 5 was significantly higher than that of the CG and tended to be higher than that of the CG during the observation period. Oct3/4 mRNA expression in the EG was significantly higher than that of the CG at day 7.

Conclusions: The current experimental model demonstrated the promotion of the survival of DPSCs and their differentiation into odontoblast-like cells (OBLCs). Thus, the use of this model is expected to clarify the crosstalk mechanism between immune cells, including macrophages and dendritic cells, and DPSCs with regards to pulpal healing after tooth replantation. It also provides insight into the differentiation process of DPSCs into OBLCs.

Keywords: Dental pulp; Mice; Odontoblast; Revascularization; Tooth replantation.