Indigenous Australian grass seeds as grains: macrostructure, microstructure and histochemistry

Farkhondeh Abedi; Claudia Keitel; Ali Khoddami; Salla Marttila; Angela L Pattison; Thomas H Roberts

doi:10.1093/aobpla/plad071

Indigenous Australian grass seeds as grains: macrostructure, microstructure and histochemistry

AoB Plants. 2023 Nov 1;15(6):plad071. doi: 10.1093/aobpla/plad071. eCollection 2023 Dec.

Authors

Farkhondeh Abedi¹, Claudia Keitel^{2

3}, Ali Khoddami^{1

3}, Salla Marttila⁴, Angela L Pattison^{3

5}, Thomas H Roberts¹

Affiliations

¹ School of Life and Environmental Sciences, University of Sydney, Camperdown, NSW 2006, Australia.
² School of Life and Environmental Sciences, University of Sydney, Camden, NSW 2570, Australia.
³ Sydney Institute of Agriculture, University of Sydney, Camperdown, NSW 2006, Australia.
⁴ Department of Plant Protection Biology, Resistance Biology Unit, Swedish University of Agricultural Sciences, 23053 Alnarp, Sweden.
⁵ School of Life and Environmental Sciences, University of Sydney, Narrabri NSW 2390, Australia.

Abstract

Utilization of grains of local grasses by Australia's First Nations people for food and connection to Country has largely been lost due to colonization. Native Australian grain production has the potential to deliver environmental, economic, nutritional and cultural benefits to First Nations people and the wider community. Revitalization of the native grain food system can only be achieved if relevant properties of the grains are elucidated. This study aimed to characterize the grain structure and histochemistry of four Australian native grasses: Dactyloctenium radulans (Button Grass), Astrebla lappacea (Curly Mitchell Grass), Panicum decompositum (Native Millet) and Microlaena stipoides (Weeping Grass). For these species, as well as wheat and sorghum, whole-grain images were obtained via stereo microscopy, starch and the embryo were visualized, and sections of fixed grains were imaged via bright-field and fluorescence microscopy. The shape, size and colour of the whole native grains varied between the species. The aleurone layer was one-cell thick in the native species, as in the domesticated grains, except for Weeping Grass, which had a two-cell-thick aleurone. In the native grains, endosperm cell walls appeared thinner than in wheat and sorghum. Starch granules in Button Grass, Curly Mitchell Grass and Native Millet were found mainly in the central region of the starchy endosperm, with very few granules in the sub-aleurone layer, whereas Weeping Grass had abundant starch in the sub-aleurone. Protein appeared most abundant in the aleurone and sub-aleurone layers of the native grains, although in Button Grass, the starchy endosperm was observed to be rich in protein, as in wheat and sorghum. As a proportion of the whole grain, the embryo was larger in the native species than in wheat. The differences found in the grain properties among the four native Australian species have important implications for the agri-food industry in a changing climate.

Keywords: Aleurone; Astrebla lappacea (Curly Mitchell Grass); Dactyloctenium radulans (Button Grass); Microlaena stipoides (Weeping Grass); Panicum decompositum (Native Millet); cell wall; embryo; endosperm; fluorescence microscopy; histochemistry.