Anti-nutrient contents and methane reduction potential of medicinal plants from maize stover based diet

Girma Chalchissa; Ajebu Nurfeta; Dereje Andualem

doi:10.1016/j.heliyon.2023.e21630

Anti-nutrient contents and methane reduction potential of medicinal plants from maize stover based diet

Heliyon. 2023 Nov 4;9(11):e21630. doi: 10.1016/j.heliyon.2023.e21630. eCollection 2023 Nov.

Authors

Girma Chalchissa^{1

2}, Ajebu Nurfeta², Dereje Andualem³

Affiliations

¹ Oromia Agricultural Research Institute, Adami Tulu Agricultural Research Center, Ziway, Ethiopia.
² Hawassa University, School of Animal and Range Science, Hawassa, Ethiopia.
³ College of Agriculture and Natural Resources, Dilla University, Dilla, Ethiopia.

Abstract

Greenhouse gas emissions from Ethiopian agriculture are significantly increasing, with the largest share is from enteric fermentation and manure left on pasture. An investigation was conducted to evaluate the anti-nutrient composition and effect of commonly used medicinal plant extracts on enteric methane emission from fibrous feeds using maize stover as substrate feed. Total phenols, flavonoid, tannin and essential oil contents were analyzed using established standards. Effects of leaf extracts of Acacia nilotica, Azadirachta indica, three varieties of Cymbopogon citratus (Cymbopogon citratus-I, Cymbopogon citratus java and Cymbopogon citratus upper awash), Leucaena leucocephala, Moringa stenopetala, three varieties of Rosmarinus officinalis (Rosmarinus officinalis I, Rosmarinus officinalis II and Rosmarinus officinalis III) and Thyme schimperi, seed of three Coriandrum sativum varieties (Coriandrum sativum Batu, Coriandrum sativum Tulu and Coriandrum sativum Waltai) and root of Echinops kebericho on total gas production, digestibility and methane production of maize stover were investigated at different doses using the standard procedures. The results indicated that leaf extracts of Acacia nilotica had the highest (P < 0.001) total phenolic and total tannin contents. Compared to other evaluated plant species, all varieties of Cymbopogon citratus had the highest (P < 0.001) flavonoid content. Significantly high (P < 0.001) essential oil content was observed in Rosmarinus officinalis II than other varieties of Rosmarinus officinalis and other plant species. Significant reduction (P < 0.001) of methane production was observed with extracts of Cymbopoon citratus java (22.5 % less methane than the control) and thyme schimperi (16.7 % less methane than the control) at dose of 50 mg/kg DM. There was also significant (P < 0.001) interaction effect between plant species and dose rates at 50 mg/kg DM for both plant species. It can be concluded that the use of 50 mg/kg DM of Cymbopoon citratus java and Thyme schimperi extract to maize stover reduced methane production without negatively affecting feed digestibility. Further studies are necessary to examine the storability of the extracts in different time durations and evaluate their effects in vivo with animals.

Keywords: Crop residue; Ethiopia; Medicinal plant; anti-methanogen.