The complete chloroplast genome of Chloranthus nervosus Collett ex Hemsl. 1890 (Chloranthaceae)

Shu-Ting Yao; Yong-Bin Lu; Zhan-Jiang Zhang; Cui Li; Peng-Fei Wang; Xin-Mei Qin

doi:10.1080/23802359.2023.2278818

The complete chloroplast genome of Chloranthus nervosus Collett ex Hemsl. 1890 (Chloranthaceae)

Mitochondrial DNA B Resour. 2023 Nov 10;8(11):1224-1228. doi: 10.1080/23802359.2023.2278818. eCollection 2023.

Authors

Shu-Ting Yao^{1

2}, Yong-Bin Lu², Zhan-Jiang Zhang³, Cui Li³, Peng-Fei Wang⁴, Xin-Mei Qin²

Affiliations

¹ College of Life Sciences, Guangxi Normal University, Guilin, China.
² Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, China.
³ Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plant, Nanning, China.
⁴ Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, China.

Abstract

In this study, we sequenced and assembled the complete chloroplast genome of Chloranthus nervosus Collett ex Hemsl. 1890. The total length of the complete chloroplast sequence was found to be 158,002 bp. It consisted of a large single-copy (LSC) region of 87,127 bp, a small single-copy (SSC) region of 18,541 bp, and a pair of inverted repeat (IR) regions, each with a length of 26,167 bp. The overall GC content of the complete chloroplast genome was 38.9%, with the LSC region, SSC region, and IR regions exhibiting GC contents of 37.4%, 34.1%, and 43.1%, respectively. The annotation of the chloroplast genome revealed a total of 131 genes, comprising 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis revealed that the seven sampled species of Chloranthus were divided into two clades. Within the clade characterized by long filamentous anther connectives, C. nervosus showed the closest relation to C. japonicus. These findings validated the previous preliminary results on the phylogenetic relationships of the seven species of Chloranthus with strong support.

Keywords: Chloranthus nervosus; chloroplast genome; phylogenetic analysis.

Grants and funding

This study was supported by the Guangxi Natural Science Foundation Project [2021GXNSFAA196049; 2020GXNSFAA159151] and Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain [22-035-26].