Single-cell trapping and retrieval in open microfluidics

Tomoki Murakami; Hiroto Teratani; Dai'ichiro Aoki; Masao Noguchi; Mamiko Tsugane; Hiroaki Suzuki

doi:10.1016/j.isci.2023.108323

Single-cell trapping and retrieval in open microfluidics

iScience. 2023 Oct 24;26(11):108323. doi: 10.1016/j.isci.2023.108323. eCollection 2023 Nov 17.

Authors

Tomoki Murakami¹, Hiroto Teratani¹, Dai'ichiro Aoki², Masao Noguchi³, Mamiko Tsugane¹, Hiroaki Suzuki¹

Affiliations

¹ Department of Precision Mechanics, Graduate School of Science and Engineering, Chuo University, Kasuga 1-13-27, Bunkyo-ku, Tokyo 112-8551, Japan.
² Aeternus Co., Ltd, Minamidai 2-1-14, Fujimino, Saitama 356-0036, Japan.
³ Caravell Co., Ltd, Surugadai 1-29-39, Funabashi, Chiba 273-0862, Japan.

Abstract

Among various single-cell analysis platforms, hydrodynamic cell trapping systems remain relevant because of their versatility. Among those, deterministic hydrodynamic cell-trapping systems have received significant interest; however, their applications are limited because trapped cells are kept within the closed microchannel, thus prohibiting access to external cell-picking devices. In this study, we develop a hydrodynamic cell-trapping system in an open microfluidics architecture to allow external access to trapped cells. A technique to render only the inside of a polydimethylsiloxane (PDMS) microchannel hydrophilic is developed, which allows the precise confinement of spontaneous capillary flow in the open-type microchannel with a width on the order of several tens of micrometers. Efficient trapping of single beads and single cells is achieved, in which trapped cells can be retrieved via automated robotic pipetting. The present system can facilitate the development of new single-cell analytical systems by bridging between microfluidic devices and macro-scale apparatus used in conventional biology.

Keywords: Applied sciences; Cell biology.