Feasibility of crude F4 fimbriae extract as a vaccine candidate for preventing Escherichia coli-induced diarrhea in piglets

Luong Thi Yen Nguyet; Puey Ounjai; Kampon Kaeoket; Natharin Ngamwongsatit

doi:10.14202/vetworld.2023.2063-2070

Feasibility of crude F4 fimbriae extract as a vaccine candidate for preventing Escherichia coli-induced diarrhea in piglets

Vet World. 2023 Oct;16(10):2063-2070. doi: 10.14202/vetworld.2023.2063-2070. Epub 2023 Oct 7.

Authors

Luong Thi Yen Nguyet¹, Puey Ounjai², Kampon Kaeoket¹, Natharin Ngamwongsatit^{1

3}

Affiliations

¹ Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.
² Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
³ Laboratory of Bacteria, Veterinary Diagnostic Center, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand.

Abstract

Background and aim: Enterotoxigenic Escherichia coli (ETEC) poses a substantial risk of neonatal diarrhea and post-weaning diarrhea among piglets, with F4⁺ ETEC strains emerging as a particularly challenging issue within the pig farming industry. This study aimed to introduce a straightforward approach for generating a crude extract of F4 fimbriae that shows promise as an antigenic determinant for potential vaccination strategies.

Materials and methods: A crude F4 fimbriae extract was obtained from F4⁺ ETEC using a combination of heat shock and homogenization techniques. Subsequently, three 4-week-old piglets were immunized with a primary dose of 150 μg and a booster dose 2 weeks later. Blood samples were collected to evaluate the level of serum F4-specific antibodies using an enzyme-linked immunosorbent assay.

Results: Analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography tandem-mass spectrometry techniques unveiled crucial insights into the composition of the crude F4 fimbriae extract. Notably, a distinct prominent band (~24 kDa) was identified, corresponding to the size of FaeG, the major subunit of F4 fimbriae. Regarding antibody response, there was a remarkable disparity between the levels of serum immunoglobulin (Ig)G and IgA antibodies targeting F4 compared with other E. coli strains (F18⁺ ETEC, F41⁺ ETEC, and F4^-F18^-F41^- EC), as well as with the unvaccinated control group (p < 0.01). Specifically, the levels of IgG antibodies against other E. coli strains were also significantly higher than those observed in the unvaccinated control group (p < 0.05).

Conclusion: Our findings suggest that the crude F4 fimbriae extracts obtained using our simple extraction method induce specific immune responses against F4⁺ E. coli and stimulate cross-immunity against other E. coli strains. Therefore, our method shows potential for use in future vaccine development against diarrhea in pigs caused by E. coli.

Keywords: Escherichia coli; F4 fimbriae; diarrhea; piglets; vaccine.