Complete genome sequence of Escherichia phage iGC_PHA_EC001

Tahsin Khan; Arefeen Haider; Sezanur Rahman; Shovan Basak Moon; Imtiaz Mahamud; Shakhinur Islam Mondal; Anowara Begum; Sudhangshu Kumar Biswas; Mohammad Jubair; Mustafizur Rahman

doi:10.1128/MRA.00842-23

Complete genome sequence of Escherichia phage iGC_PHA_EC001

Microbiol Resour Announc. 2024 Jan 17;13(1):e0084223. doi: 10.1128/MRA.00842-23. Epub 2023 Nov 29.

Authors

Affiliations

¹ Genome Centre, Infectious Diseases Division, icddr,b, Mohakhali , Dhaka, Bangladesh.
² Virology Laboratory, Infectious Diseases Division, icddr,b, Mohakhali , Dhaka, Bangladesh.
³ Department of Genetic Engineering and Biotechnology, Shahjalal University of Science Technology , Sylhet, Bangladesh.
⁴ Department of Microbiology, University of Dhaka , Dhaka, Bangladesh.
⁵ Department of Biotechnology and Genetic Engineering, Islamic University , Kushtia, Bangladesh.

Abstract

Antimicrobial resistance (AMR) in bacteria poses a global health emergency due to limited treatment options. Here, we report a lytic bacteriophage belonging to Stephanstirmvirinae family against an AMR Escherichia coli (ST2089). Escherichia phage iGC_PHA_EC001 is of genus Phapecoctavirus and 148,445 bp in length, encoding 269 predicted protein-coding sequences and 10 tRNAs. The phage encodes two lytic proteins containing phage_lysozyme (PF00959.22) and cell wall hydrolase_2 (PF07486.15) as catalytic domains, respectively.

Keywords: Bangladesh; EC001; Escherichia phage; Phapecoctavirus; bacteriophages; endolysins; iPHaGE; lytic bacteriophage; phage.