High resolution visualisation of tiemannite microparticles, essential in the detoxification process of mercury in marine mammals

Abstract

The North Sea is an ecologically rich habitat for marine wildlife which has also been impacted by industrial developments and anthropogenic emissions of contaminants such as mercury. Marine mammals are particularly susceptible to mercury exposure, due to their trophic position, long lifespan, and dependence on (increasingly contaminated) aquatic prey species. To mitigate impact, marine mammals can detoxify methylmercury by binding it to selenium-containing biomolecules, creating insoluble mercury selenide granules. Here, liver, kidney, muscle, and brain samples from an adult male bottlenose dolphin (Tursiops truncatus) with known elevated mercury concentrations were analysed through scanning electron microscopy (SEM). Tiemannite (HgSe) deposits were identified in all organs, ranging from 400 nm to 5 μm in diameter, with particle size being organ-dependent. Although reported in other studies, this is the first time that the three-dimensional nature of tiemannite is captured in marine mammal tissue.

Keywords: Biomagnification; Mercury; Scanning electron microscopy; Tursiops truncatus.