The effects of noise exposure on hippocampal cognition in C57BL/6 mice via transcriptomics

Zan Zhou; Wen-Jun Jiang; Li Li; Jun-Qiang Si

doi:10.1016/j.bbrc.2023.149257

The effects of noise exposure on hippocampal cognition in C57BL/6 mice via transcriptomics

Biochem Biophys Res Commun. 2024 Jan 1:690:149257. doi: 10.1016/j.bbrc.2023.149257. Epub 2023 Nov 23.

Authors

Zan Zhou¹, Wen-Jun Jiang², Li Li³, Jun-Qiang Si⁴

Affiliations

¹ Department of Physiology, Medical College of Shihezi University, Shihezi, Xinjiang, 832000, China; Department of Physiology, Medical College of Jiaxing University, Jiaxing, Zhejiang, 314000, China; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, 832000, Xinjiang, China.
² Department of Physiology, Medical College of Jiaxing University, Jiaxing, Zhejiang, 314000, China; Department of Physiology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310051, China.
³ Department of Physiology, Medical College of Jiaxing University, Jiaxing, Zhejiang, 314000, China. Electronic address: lily7588@163.com.
⁴ Department of Physiology, Medical College of Shihezi University, Shihezi, Xinjiang, 832000, China; The Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi, 832000, Xinjiang, China. Electronic address: sijunqiang@shzu.edu.cn.

PMID: 38016245
DOI: 10.1016/j.bbrc.2023.149257

Abstract

Background: Noise is an important environmental stressor in the industrialized world and has received increasing attention in recent years. Although epidemiological research has extensively demonstrated the relationship between noise and cognitive impairment, the specific molecular mechanisms and targets remain to be fully explored and understood.

Methods: To address this issue, 5-month-old C57BL/6 mice were divided into two groups, with one group exposed to white noise at 98 dB. The effects of noise on cognition in mice were investigated through molecular biology and behavioral experiments. Subsequently, transcriptomic sequencing of the hippocampus in both groups of mice was performed and enrichment analysis of differentially expressed genes (DEGs) was conducted using KEGG and GO databases. Furthermore, LASSO analysis was used to further narrow down the relevant DEGs, followed by enrichment analysis of these genes using KEGG and GO databases. The DEGs were further validated by rt-qPCR.

Results: Following noise exposure, the hippocampus levels of inflammation-related factors increased, the phosphorylation of Tau protein increased, the postsynaptic density protein decreased, the number of Nissl bodies decreased, and cell shrinkage in the hippocampus increased. Moreover, the behavioral experiments manifest characteristics indicative of a decline in cognitive.A total of 472 DEGs were identified through transcriptomic analysis, and seven relevant genes were screened by the LASSO algorithm, which were further validated by PCR to confirm their consistency with the omics results.

Conclusion: In conclusion, noise exposure affects cognitive function in mice through multiple pathways, and the omics results provide new evidence for the cognitive impairment induced by noise exposure.

Keywords: Cognitive disorder; Hippocampus; LASSO analysis; Noise expose; Transcriptomic.

MeSH terms

Animals
Cognition
Cognitive Dysfunction*
Gene Expression Profiling*
Hippocampus / metabolism
Mice
Mice, Inbred C57BL