Livestock dung rather than biochar enhances the anaerobic methane oxidation in grassland soils

Qingzhou Zhao; Xiao Luo; Yanfen Wang; Zhihong Xu; Zhisheng Yu

doi:10.1016/j.scitotenv.2023.168861

Livestock dung rather than biochar enhances the anaerobic methane oxidation in grassland soils

Sci Total Environ. 2024 Feb 20:912:168861. doi: 10.1016/j.scitotenv.2023.168861. Epub 2023 Nov 25.

Authors

Qingzhou Zhao¹, Xiao Luo², Yanfen Wang³, Zhihong Xu⁴, Zhisheng Yu⁵

Affiliations

¹ College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China; College of Urban and Environmental Science, Peking University, Beijing 100871, PR China; Center of Planetary Health and Food Security, Griffith University, Nathan, QLD 4111, Australia.
² Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101408, PR China.
³ College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China.
⁴ Center of Planetary Health and Food Security, Griffith University, Nathan, QLD 4111, Australia; School of Environment and Science, Griffith University, Nathan, QLD 4111, Australia.
⁵ College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China; RCEES-IMCAS-UCAS Joint-Lab of Microbial Technology for Environmental Science, Beijing 100085, PR China. Electronic address: yuzs@ucas.ac.cn.

PMID: 38013103
DOI: 10.1016/j.scitotenv.2023.168861

Abstract

The terrestrial anaerobic methane oxidation (AOM) coupled with denitrification is considered to be an important link in the "cryptic cycle of methane". However, it remains uncertain how land use activity such as biochar and livestock dung amendments regulate the AOM in grassland. Here, we incubated soils with biochar and dung amendments in microcosms to monitor the AOM activity and quantified the maker genes of anaerobic methanotrophs and their potential syntrophs. Dung enhanced the AOM mediated by Candidatus Methylomirabilis oxyfera and stimulated denitrifying bacteria and anammox growths as well. The biochar amendment inhibited AOM due to the trapping of NO₃^- and NO₂^-. Our study raised the possibility that anthropogenic activity can regulate AOM through porosity alteration and substrate limitation.

Keywords: Anaerobic methane oxidation; Biochar amendment; Denitrification; Dung amendment.

MeSH terms

Anaerobiosis
Animals
Archaea* / genetics
Bacteria / genetics
Charcoal*
Grassland
Livestock*
Methane
Oxidation-Reduction

Substances

biochar
Methane
Charcoal