Design and fabrication of a magnetic nanobiocomposite based on flaxseed mucilage hydrogel and silk fibroin for biomedical and in-vitro hyperthermia applications

Fateme Radinekiyan; Reza Eivazzadeh-Keihan; Mohammad Reza Naimi-Jamal; Hooman Aghamirza Moghim Aliabadi; Milad Salimi Bani; Shirin Shojaei; Ali Maleki

doi:10.1038/s41598-023-46445-w

Design and fabrication of a magnetic nanobiocomposite based on flaxseed mucilage hydrogel and silk fibroin for biomedical and in-vitro hyperthermia applications

Sci Rep. 2023 Nov 27;13(1):20845. doi: 10.1038/s41598-023-46445-w.

Authors

Fateme Radinekiyan^{1

2}, Reza Eivazzadeh-Keihan², Mohammad Reza Naimi-Jamal³, Hooman Aghamirza Moghim Aliabadi⁴, Milad Salimi Bani⁵, Shirin Shojaei⁶, Ali Maleki⁷

Affiliations

¹ Research Laboratory of Green Organic Synthesis and Polymers, Department of Chemistry, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran.
² Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran.
³ Research Laboratory of Green Organic Synthesis and Polymers, Department of Chemistry, Iran University of Science and Technology, P.O. Box 16846-13114, Tehran, Iran. naimi@iust.ac.ir.
⁴ Advanced Chemical Studies Lab, Department of Chemistry, K. N. Toosi University of Technology, Tehran, Iran.
⁵ Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran.
⁶ Medical School of Pharmacy, Nanotechnology Department, Kermanshah University of Medical Science, Kermanshah, Iran.
⁷ Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran. maleki@iust.ac.ir.

Abstract

In this research work, a magnetic nanobiocomposite is designed and presented based on the extraction of flaxseed mucilage hydrogel, silk fibroin (SF), and Fe₃O₄ magnetic nanoparticles (Fe₃O₄ MNPs). The physiochemical features of magnetic flaxseed mucilage hydrogel/SF nanobiocomposite are evaluated by FT-IR, EDX, FE-SEM, TEM, XRD, VSM, and TG technical analyses. In addition to chemical characterization, given its natural-based composition, the in-vitro cytotoxicity and hemolysis assays are studied and the results are considerable. Following the use of highest concentration of magnetic flaxseed mucilage hydrogel/SF nanobiocomposite (1.75 mg/mL) and the cell viability percentage of two different cell lines including normal HEK293T cells (95.73%, 96.19%) and breast cancer BT549 cells (87.32%, 86.9%) in 2 and 3 days, it can be inferred that this magnetic nanobiocomposite is biocompatible with HEK293T cells and can inhibit the growth of BT549 cell lines. Besides, observing less than 5% of hemolytic effect can confirm its hemocompatibility. Furthermore, the high specific absorption rate value (107.8 W/g) at 200 kHz is generated by a determined concentration of this nanobiocomposite (1 mg/mL). According to these biological assays, this magnetic responsive cytocompatible composite can be contemplated as a high-potent substrate for further biomedical applications like magnetic hyperthermia treatment and tissue engineering.

MeSH terms

Biocompatible Materials / chemistry
Fibroins* / chemistry
Flax*
HEK293 Cells
Humans
Hydrogels / chemistry
Hyperthermia, Induced*
Magnetic Phenomena
Silk / chemistry
Spectroscopy, Fourier Transform Infrared

Substances

Fibroins
Hydrogels
Biocompatible Materials
Silk