Simultaneous determination of 17 regulated and non-regulated Fusarium mycotoxins co-occurring in foodstuffs by UPLC-MS/MS with solid-phase extraction

Abstract

Fusarium species produce numerous mycotoxins known to co-occur in food. While some of these mycotoxins (e.g., deoxynivalenol, fumonisins) are regulated in several countries, others are non-regulated (e.g., nivalenol, beauvericin). In this study, UPLC-MS/MS with solid-phase extraction cleanup was used to determine 17 Fusarium mycotoxins (FTs) simultaneously. The method showed excellent performance in terms of linearity (R² > 0.99), LOD (<1.2 μg/kg), LOQ (<3.6 μg/kg), accuracy (70.0-116.3 %), repeatability (<15.7 %), reproducibility (<25.3 %), and expanded uncertainty (<41.7 %). The validated method was successfully applied to 198 marketed food samples collected in South Korea. Of the tested samples, 79 % were contaminated with at least one FT. Job's tears showed the highest prevalence of 14 FTs, and sorghum had the highest total FTs level (3.03 mg/kg). The results suggest that this method can be used for the simultaneous analysis of 17 FTs in food samples, which would serve as crucial information for risk management.

Keywords: Co-occurrence; Correlation; Fusarium mycotoxin; Method validation; Simultaneous determination.