Challenges in recombinant brain-derived neurotrophic factor production

Éllen F Rodrigues; Ana L Fachin; Mozart Marins; Rodrigo G Stabeli; Renê O Beleboni

doi:10.1016/j.tibtech.2023.11.003

Challenges in recombinant brain-derived neurotrophic factor production

Trends Biotechnol. 2023 Nov 25:S0167-7799(23)00325-6. doi: 10.1016/j.tibtech.2023.11.003. Online ahead of print.

Authors

Éllen F Rodrigues¹, Ana L Fachin², Mozart Marins³, Rodrigo G Stabeli⁴, Renê O Beleboni⁵

Affiliations

¹ Biotechnology Unit, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil.
² Biotechnology Unit, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil; Medicine School, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil.
³ Biotechnology Unit, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil; Medicine School, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil; Pharmacy School, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil.
⁴ Oswaldo Cruz Foundation/Fiocruz-SP, Ribeirão Preto, SP, Brazil; Department of Medicine, Federal University of São Carlos, São Carlos, SP, Brazil.
⁵ Biotechnology Unit, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil; Medicine School, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil; Pharmacy School, University of Ribeirão Preto/UNAERP, Ribeirão Preto, SP, Brazil. Electronic address: rbeleboni@unaerp.br.

PMID: 38008688
DOI: 10.1016/j.tibtech.2023.11.003

Abstract

Brain-derived neurotrophic factor (BDNF) is a neurotrophin of marked commercial, scientific, diagnostic, and therapeutic interest. The preservation of its structural cystine-knot is the main challenge in its industrial production. A suitable expression system is critical to achieve the most efficient production of bioactive and stable BDNF for pharmaceutical purposes.

Keywords: expression systems; pharmaceutical biotechnology; recombinant proteins.