A novel device for the determination of liposome/water partition coefficients

Radek Jerga; Veronika Brablecová; Veronika Talášková; Hana Tomková; Jitka Součková; Petr Barták; Jana Skopalová

doi:10.1016/j.talanta.2023.125434

A novel device for the determination of liposome/water partition coefficients

Talanta. 2024 Mar 1:269:125434. doi: 10.1016/j.talanta.2023.125434. Epub 2023 Nov 19.

Authors

Radek Jerga¹, Veronika Brablecová¹, Veronika Talášková¹, Hana Tomková¹, Jitka Součková¹, Petr Barták¹, Jana Skopalová²

Affiliations

¹ Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic.
² Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 1192/12, 771 46, Olomouc, Czech Republic. Electronic address: jana.skopalova@upol.cz.

PMID: 38008025
DOI: 10.1016/j.talanta.2023.125434

Abstract

A novel, cheap and easy-to-construct device and a simple method for partition coefficient determination in liposome/water system based on modified equilibrium dialysis have been developed. The device consists of two vials separated by a semi-permeable membrane, through which the free form of a low molecular weight substance is transported by shaking assisted diffusion. Five test substances, eugenol, carvacrol, thymol, 4-hydroxybenzyl alcohol (4-HBA) and butylparaben were analyzed after equilibration in aqueous phase by three methods, HPLC-UV, GC-MS and DPV with comparable results. This shows the possibility of using the proposed method in any laboratory with any equipment capable of analyzing the substance under study. The liposome/water partition coefficients (log P_l/w) determined for eugenol (2.39), thymol (2.83), carvacrol (2.78) and butylparaben (3.30) are consistent with previously published data. A strong effect of NaCl on the liposome/water partition coefficient was observed. The value of log P_l/w = 1.06 determined for 4-HBA in the presence of 0.15 mol L^-1 NaCl in the partitioning liposomal system was considerably lower than in the absence of the salt (log P_l/w = 2.06). The developed method was used to determine the partition coefficient of morphine in liposome/water system without NaCl (log P_l/w = 2.65) under given conditions.

Keywords: 4-Hydroxybenzyl alcohol; Asolectin; Butylparaben; Liposome/water partition coefficient; Morphine; Phenolic compounds.