Essential oil components of turmeric inhibit hepatic lipidification and liver fibrosis in a diet-induced NASH model rats

Yukari Watanabe; Hitoshi Watanabe; Sarasa Kogure; Yuri Tanioka; Jun Yamauchi; Tadasu Furusho

doi:10.1038/s41598-023-47097-6

Essential oil components of turmeric inhibit hepatic lipidification and liver fibrosis in a diet-induced NASH model rats

Sci Rep. 2023 Nov 25;13(1):20742. doi: 10.1038/s41598-023-47097-6.

Authors

Yukari Watanabe¹, Hitoshi Watanabe², Sarasa Kogure³, Yuri Tanioka^{1

2

3}, Jun Yamauchi^{2

3}, Tadasu Furusho^{4

5

6}

Affiliations

¹ Department of Ecological Symbiotic Science, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.
² Department of International Food and Agricultural Science, Faculty of International Agriculture and Food Studies, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.
³ Department of International Food and Agricultural Science, Faculty of International Agriculture and Food Studies, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.
⁴ Department of Ecological Symbiotic Science, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan. tfurusho@nodai.ac.jp.
⁵ Department of International Food and Agricultural Science, Faculty of International Agriculture and Food Studies, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan. tfurusho@nodai.ac.jp.
⁶ Department of International Food and Agricultural Science, Faculty of International Agriculture and Food Studies, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan. tfurusho@nodai.ac.jp.

Abstract

In this study, the fraction extracted from turmeric powder with 50% ethanol and fractionated with n-hexane were administered to diet-induced NASH model rats. NASH model was prepared with SD rats by feeding an originally designed choline-deficient, high-fat, high-fructose (HFF-CD) diet for 10 weeks. To the HFF-CD diet, hexane fraction and 50% ethanol fraction after hexane fractionation were added at 100 mg/kg body weight. 10 weeks later, blood samples and liver were collected for the following parameters: lipid weights, serum ALT, AST, TG, liver TG, TBARS levels, lipid metabolism-related gene expression and histopathological examination of the liver. As the results, the hexane fraction and 50% ethanol fraction showed a decrease in lipid weight, a decrease in hepatic TG, and activation of PPAR-α in the lipid metabolism-related gene test. These results suggest that the hexane fraction of turmeric has an inhibitory effect on fat accumulation in the liver by promoting lipid metabolism in NASH model rats.

MeSH terms

Animals
Curcuma
Diet, High-Fat / adverse effects
Ethanol / pharmacology
Hexanes / metabolism
Lipid Metabolism / genetics
Lipids / pharmacology
Liver / metabolism
Liver Cirrhosis / pathology
Non-alcoholic Fatty Liver Disease* / drug therapy
Non-alcoholic Fatty Liver Disease* / etiology
Non-alcoholic Fatty Liver Disease* / metabolism
Rats
Rats, Sprague-Dawley

Substances

n-hexane
Hexanes
Lipids
Ethanol