Developing Wolbachia-Infected Cell Lines from Insects

Mukund Madhav; Peter James

doi:10.1007/978-1-0716-3553-7_8

Developing Wolbachia-Infected Cell Lines from Insects

Methods Mol Biol. 2024:2739:137-145. doi: 10.1007/978-1-0716-3553-7_8.

Authors

Mukund Madhav^{1

2}, Peter James³

Affiliations

¹ Liverpool School of Tropical Medicine, Liverpool, UK.
² Health & Biosecurity, Commonwealth Scientific and Industrial Research Organisation, Geelong, Australia.
³ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia. p.james1@uq.edu.au.

PMID: 38006549
DOI: 10.1007/978-1-0716-3553-7_8

Abstract

Wolbachia, an intracellular bacterium of arthropods, is an ideal candidate for use in the biological control of insect pests. The inability of Wolbachia to grow in the extracellular environment requires maintenance in live insects or insect cell cultures. Growing and adapting high-density Wolbachia in the targeted host cells improves the possibility of cross-species transinfection. Here, we describe a method for the establishment of a primary cell culture from insect embryos and its transinfection with Wolbachia.

Keywords: Agricultural pest; Biocontrol; Ectoparasite; HIE-18 cell line; Haematobia spp..

MeSH terms

Animals
Cell Line
Insecta
Wolbachia*