Single-Cell PET Imaging and Tracking

Kyung Oh Jung; Guillem Pratx

doi:10.1007/978-1-0716-3499-8_19

Single-Cell PET Imaging and Tracking

Methods Mol Biol. 2024:2729:331-340. doi: 10.1007/978-1-0716-3499-8_19.

Authors

Kyung Oh Jung^{1

2}, Guillem Pratx³

Affiliations

¹ Department of Radiation Oncology, Stanford University, Stanford, CA, USA.
² Department of Anatomy, Chung-Ang University, Seoul, South Korea.
³ Department of Radiation Oncology, Stanford University, Stanford, CA, USA. pratx@stanford.edu.

PMID: 38006505
DOI: 10.1007/978-1-0716-3499-8_19

Abstract

Positron emission tomography (PET) is one of the most sensitive whole-body molecular imaging techniques available in the clinic, able to detect picomolar levels of probe. As such, it was recently demonstrated that PET could also be used to track single radiolabeled cells in small animals. In this protocol, we present detailed procedures for radiolabeling cells using mesoporous silica nanoparticles (MSNs) and for tracking these cells in real time using in vivo PET. This includes static imaging of single cells as well as dynamic tracking of moving cells directly from the list-mode data. The protocol provides detailed instructions and examples for each step.

Keywords: Cancer metastasis; Cell tracking; Nanotechnology; Positron emission tomography.

MeSH terms

Animals
Nanoparticles*
Positron-Emission Tomography* / methods
Whole Body Imaging