Imaging the Tumor Antioxidant Response with [18F]FSPG PET

Oskar Vilhelmsson Timmermand; Timothy H Witney

doi:10.1007/978-1-0716-3499-8_14

Imaging the Tumor Antioxidant Response with [¹⁸F]FSPG PET

Methods Mol Biol. 2024:2729:233-249. doi: 10.1007/978-1-0716-3499-8_14.

Authors

Oskar Vilhelmsson Timmermand¹, Timothy H Witney²

Affiliations

¹ School of Biomedical Engineering & Imaging Sciences, King's College London, London, UK.
² School of Biomedical Engineering & Imaging Sciences, King's College London, London, UK. tim.witney@kcl.ac.uk.

PMID: 38006500
DOI: 10.1007/978-1-0716-3499-8_14

Abstract

(4S)-4-(3-[¹⁸F]Fluoropropyl)-L-glutamic acid ([¹⁸F]FSPG) is a flourine-18 labeled glutamate analog that enables the noninvasive in vivo imaging of cellular redox status. [¹⁸F]FSPG is transported across the cell membrane by the cystine/glutamate antiporter, system x_c^-, whose expression is upregulated in multiple cancer types. The requirement of cystine for the biosynthesis of glutathione, a major antioxidant, connects [¹⁸F]FSPG tissue retention to the intracellular redox response via system x_c^- activity. We herein describe the use of [¹⁸F]FSPG positron emission tomography (PET) to image the tumor antioxidant response and highlight key methodological considerations.

Keywords: (4S)-4-(3-[18F]Fluoropropyl)-L-glutamic acid; Cystine; Glutamate; Glutathione; PET; ROS; [18F]FSPG; xc−.

MeSH terms

Antioxidants*
Cystine
Glutamic Acid
Humans
Neoplasms* / diagnostic imaging
Positron-Emission Tomography / methods

Substances

Antioxidants
Cystine
Glutamic Acid