Analysis of Auxin-Encoding Gene Family in Vigna radiata and It's Cross-Species Expression Modulating Waterlogging Tolerance in Wild Vigna umbellata

Chandra Mohan Singh; Shalini Purwar; Akhilesh Kumar Singh; Bhupendra Kumar Singh; Mukul Kumar; Hitesh Kumar; Aditya Pratap; Awdhesh Kumar Mishra; Kwang-Hyun Baek

doi:10.3390/plants12223858

Analysis of Auxin-Encoding Gene Family in Vigna radiata and It's Cross-Species Expression Modulating Waterlogging Tolerance in Wild Vigna umbellata

Plants (Basel). 2023 Nov 15;12(22):3858. doi: 10.3390/plants12223858.

Authors

Chandra Mohan Singh¹, Shalini Purwar², Akhilesh Kumar Singh³, Bhupendra Kumar Singh⁴, Mukul Kumar¹, Hitesh Kumar¹, Aditya Pratap⁵, Awdhesh Kumar Mishra⁶, Kwang-Hyun Baek⁶

Affiliations

¹ Department of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda 210 001, India.
² Department of Basic and Social Sciences, Banda University of Agriculture and Technology, Banda 210 001, India.
³ Department of Plant Protection, Banda University of Agriculture and Technology, Banda 210 001, India.
⁴ Department of Entomology, Banda University of Agriculture and Technology, Banda 210 001, India.
⁵ Crop Improvement Division, ICAR-Indian Institute of Pulses Research, Kanpur 208 024, India.
⁶ Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.

Abstract

Mungbean is known to be susceptible to waterlogging (WL) stress. Some of the wild species have the potential to tolerate this through various physiological and molecular mechanisms. Auxin Response Factor (ARF) and Auxin/Indole Acetic Acid (AUX/IAA), an early responsive gene family, has multiple functions in growth, development, and stress tolerance. Here, we report the first comprehensive analysis of the ARF and AUX/IAA gene family in mungbean. A total of 26 ARF and 19 AUX/IAA genes were identified from the mungbean genome. The ARF and AUX/IAA candidates were clearly grouped into two major clades. Further, the subgrouping within the major clades indicated the presence of significant diversity. The gene structure, motif analysis, and protein characterization provided the clue for further fundamental research. Out of the10 selected candidate genes, VrARF-5, VrARF-11, VrARF-25, and VrAUX/IAA-9 were found to significantly multiple-fold gene expression in the hypocotyl region of WL-tolerant wild relatives (PRR 2008-2) provides new insight into a role in the induction of lateral root formation under WL stress. The analysis provides an insight into the structural diversity of ARF and AUX/IAA genes in mungbean. These results increase our understanding of ARF and AUX/IAA genes and therefore offer robust information for functional investigations, which can be taken up in the future and will form a foundation for improving tolerance against waterlogging stress.

Keywords: ARF; Vigna umbellata; auxin-encoding genes; crop wild relatives; seedling waterlogging.

Grants and funding

The present work was financially supported by the Ministry of Agriculture, Agriculture Education and Research, Govt. of Uttar Pradesh, under the projects “Center of Excellence in Dryland Agriculture (CEDA/2018-23)” and “Establishment of Central Laboratory Facility under RKVY (1132/12-3-2020-100(09)/2018TC)”.