Validation of dried blood spot for serological diagnosis of Hepatitis B and C: a multicentric study

Arati Mane; Reshu Agarwal; Meenu Bajpai; Suvarna Sane; Pallavi Vidhate; Partha Rakshit; Preeti Madan; Hema Gogia; Priya Abraham; Sandhya Kabra; Ekta Gupta

doi:10.1016/j.diagmicrobio.2023.116108

Validation of dried blood spot for serological diagnosis of Hepatitis B and C: a multicentric study

Diagn Microbiol Infect Dis. 2024 Feb;108(2):116108. doi: 10.1016/j.diagmicrobio.2023.116108. Epub 2023 Oct 15.

Authors

Arati Mane¹, Reshu Agarwal², Meenu Bajpai³, Suvarna Sane⁴, Pallavi Vidhate⁴, Partha Rakshit⁵, Preeti Madan⁵, Hema Gogia⁵, Priya Abraham⁶, Sandhya Kabra⁵, Ekta Gupta⁷

Affiliations

¹ Division of Microbiology, ICMR- NARI, Pune, India.
² Department of Clinical Virology, Institute of Liver and Biliary Sciences, New Delhi, India.
³ Department of Transfusion Medicine, Institute of Liver and Biliary Sciences, New Delhi, India.
⁴ Division of Epidemiology and Biostatistics, ICMR- NARI, Pune, India.
⁵ National Centre for Disease Control, New Delhi, India.
⁶ Department of Virology, Christian Medical College, Vellore, Tamil Nadu, India.
⁷ Department of Clinical Virology, Institute of Liver and Biliary Sciences, New Delhi, India. Electronic address: ektagaurisha@gmail.com.

PMID: 38000329
DOI: 10.1016/j.diagmicrobio.2023.116108

Abstract

The present study aimed to determine diagnostic performance of dried blood spot (DBS) for the detection of Hepatitis B surface antigen (HBsAg) and Hepatitis C virus antibodies (anti-HCV) using CLIA at 3 different laboratories across India. DBS can serve as a simple and convenient alternative to plasma/serum for HBsAg detection. However for anti-HCV, site-specific validation of the assay is warranted.

Keywords: Chemiluminescence; Dried blood spot; Finger-prick; Hepatitis B; Hepatitis C.

Publication types

Multicenter Study

MeSH terms

Dried Blood Spot Testing
Hepacivirus
Hepatitis B Surface Antigens
Hepatitis B* / diagnosis
Hepatitis C Antibodies
Hepatitis C* / diagnosis
Humans
Sensitivity and Specificity

Substances

Hepatitis B Surface Antigens
Hepatitis C Antibodies