Effects of chlorination on the survival of sewage bacteria in seawater microcosms

Abstract

Chlorination is a commonly used disinfection method in sewage treatment process. However, resistant bacteria may survive chlorination and enter the receiving aquatic environment upon effluent discharge. There has been limited research on the effects of chlorination on bacterial survival in seawater. To address this knowledge gap, microcosm experiments were conducted to simulate the discharge of chlorinated effluents into coastal seawater. The results revealed that bacterial communities in seawater-based effluents survived better in seawater than those in freshwater-based effluents. High chlorine dosages could significantly reduce the viable bacterial populations and their chance of regrowth in seawater. Additionally, faecal indicator bacteria (FIB) that entered the viable but non-culturable (VBNC) state under chlorination tended to persist in the VBNC state without resuscitation during seawater incubation. Because of the prevalence of VBNC indicator bacteria, qPCR quantification of FIB was more effective than conventional culture-based methods in tracing viable pathogenic chlorine-resistant bacteria, although the correlation strength varied depending on the type of effluent. This study sheds light on how chlorine dosages and the intrinsic properties of effluents affect bacterial survival in seawater and highlights the potential and limitations of using FIB in monitoring the health risks associated with the discharge of chlorinated effluents.