Multi-spectroscopic and thermodynamic profiles on HSA binding of Cassia fistula leaf based potential antibacterial and anticancer silver nanoparticles

Maidul Beg; Anukul Maji; Mt Nasima Aktara; Somenath Kundu; Samaresh Paria; Basudev Shit; Asima Dhal; Md Maidul Islam; Maidul Hossain

doi:10.1080/07391102.2023.2283148

Multi-spectroscopic and thermodynamic profiles on HSA binding of Cassia fistula leaf based potential antibacterial and anticancer silver nanoparticles

J Biomol Struct Dyn. 2023 Nov 21:1-13. doi: 10.1080/07391102.2023.2283148. Online ahead of print.

Authors

Maidul Beg¹, Anukul Maji¹, Mt Nasima Aktara¹, Somenath Kundu¹, Samaresh Paria¹, Basudev Shit¹, Asima Dhal¹, Md Maidul Islam², Maidul Hossain¹

Affiliations

¹ Department of Chemistry, Vidyasagar University, Midnapore, India.
² Department of Chemistry, Aliah University, Kolkata,India.

PMID: 37990582
DOI: 10.1080/07391102.2023.2283148

Abstract

Here, a simple, one step, lucrative and green synthesis of Cassia fistula leaf extract inspired antibacterial silver nanoparticles (CF-SNPs) was provided. Characterization of these CF-SNPs were achieved by using various spectroscopic techniques for instance Ultraviolet Visible (UV-Vis) Spectroscopy, Fourier-Transform Infrared (FTIR) Spectroscopy, Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX). The effective antibacterial action of the CF-SNPs was checked against Escherichia coli (E. Coli) DH5-Alpha where MIC was 1.6 nM. Anticancer dynamism of the CF-SNPs was also tested in opposition to skin melanoma, A375 cell lines in which 4.4 nM was IC₅₀. The binding proneness of HSA towards CF-SNPs was investigated by means of UV-Vis Spectroscopy, Fluorescence Spectroscopy, Time Resolved Fluorescence Spectroscopy, Circular Dichroism (CD) Spectroscopy, Dynamic Light Scattering, and Isothermal Titration Colorimetry (ITC). CD spectroscopy established minor secondary structural exchange of HSA in HSA-CF-SNPs complex. ITC and Time Resolved Fluorescence Spectroscopy verified the static type quenching mechanism involved in HSA-CF-SNPs complex. The binding constant was 3.45 × 10⁸ M^-1 at 298.15K from ITC study. The thermodynamic parameters showed that the interaction was occurred spontaneously by the hydrophilic forces and hydrogen bonding.Communicated by Ramaswamy H. Sarma.

Keywords: Green synthesis; antibacterial activity; anticancer activity; circular dichroism; isothermal titration colorimetry; silver nanoparticle.