Males are often predicted to prefer virgin over non-virgin females because of the reduced risk of sperm competition. Does this prediction hold across studies? Our systematic meta-analysis of 138 studies, mainly conducted in invertebrates, confirms that males generally prefer virgin females. However, males preferred virgin females even in species with last male sperm precedence, suggesting that sperm competition alone does not drive male preferences. Furthermore, our results suggest that males may reject mated females even when no alternative exists. Preference for virgins is unlikely to influence female reproductive success since virginity cannot be selected for, but strong preference for virgin females could swamp or reinforce selection on other traits. Our results add to growing evidence that males are not indiscriminate in mating. However, given the unexplained heterogeneity in effect sizes, we urge caution in assuming that males will prefer virgins and recommend considering the natural context of mating decisions.
Keywords: female mating status; male preference; mate choice; meta-analysis.
© 2023 The Authors. Ecology Letters published by John Wiley & Sons Ltd.