In this study, the persistent effects of embryonic exposure to fenbuconazole (FBZ), a triazole fungicide, on neurobehaviour in zebrafish were investigated. After exposure of fertilized eggs to FBZ for 72 h (h), the larvae were cultured to adulthood in clean water. In adult zebrafish embryonically exposed to 50 and 500 ng L-1 FBZ, the ratio of brain weight/body weight was significantly decreased, and the number of apoptotic cells in the brain was significantly increased, accompanied by upregulated protein levels of P53 and downregulated levels of BCL2. The novel tank test showed a significant reduction in the moved distance and speed, and a longer period of adaptation to new environments in the 500 ng L-1 group. The social preference experiment showed impaired social interaction behaviour and reduced time of aggregation in the 500 ng L-1 group. Increased dopamine and norepinephrine levels in the brain might be responsible for this anxiety-like behaviour. In addition to upregulated protein levels of tyrosine hydroxylase and β2-adrenoceptor, the transcription of genes related to dopamine and norepinephrine synthesis in the brain such as th1, th2, ddc, drd1b, dat, and dbh, was increased. The methylation levels of related genes were reduced, which were matched with their increased transcriptional levels. These results demonstrate that embryonic FBZ exposure might cause persistent neurotoxicity in adulthood, which suggests the rational cautious use of FBZ.
Keywords: Early-life exposure; Mechanism; Neurobehaviour; Triazole fungicide; Zebrafish.
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