Rapid analysis of dichlorvos via releasing the phosphate core

Lihua Wu; Jing Guo; Yuxin Chen; Yu-Xin Ye; Yuan-Jun Tong; Fang Zhu; Jianqiao Xu; Gangfeng Ouyang

doi:10.1016/j.talanta.2023.125404

Rapid analysis of dichlorvos via releasing the phosphate core

Talanta. 2024 Mar 1:269:125404. doi: 10.1016/j.talanta.2023.125404. Epub 2023 Nov 14.

Authors

Lihua Wu¹, Jing Guo¹, Yuxin Chen², Yu-Xin Ye³, Yuan-Jun Tong⁴, Fang Zhu¹, Jianqiao Xu⁵, Gangfeng Ouyang¹

Affiliations

¹ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, IGCME, Sun Yat-sen University, Guangzhou, 510006, China.
² Instrumental Analysis & Research Center, Sun Yat-sen University, Guangzhou, 510275, China.
³ School of Chemical Engineering and Technology, IGCME, Sun Yat-sen University, Zhuhai, Guangdong, 519082, China.
⁴ Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 610031, Sichuan, China. Electronic address: tongyj@swjtu.edu.cn.
⁵ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, IGCME, Sun Yat-sen University, Guangzhou, 510006, China. Electronic address: xujq27@mail.sysu.edu.cn.

PMID: 37980819
DOI: 10.1016/j.talanta.2023.125404

Abstract

Monitoring the residual dichlorvos (O,O-dimethyl-O-2,2-dichlorovinylphosphate, DDVP) in food has received extensive attention owing to its large consumption in agriculture. However, the previous sensing methods are not time-efficient enough due to the long incubation time for enzyme inhibition (tens of minutes to hours) or bottlenecked by the complicated procedures for senor fabrication. Herein, a novel sensing strategy is proposed based on the hydrolysis of DDVP into PO₄^3-. By using alkaline phosphatase for hydrolysis, a certain portion of DDVP was transformed to PO₄^3- within only 8 min. Then, the released PO₄^3- was detected by a fluorescent terbium metal-organic framework (Tb-MOF). The coordination of the naked P-O groups to the metal nodes of the Tb-MOF disturbed the antenna effects of its ligands. Thus, DDVP was quantified by the decrease of the fluorescence of Tb ions. Based on this method, DDVP residues on plum surfaces were collected by swabs and successfully detected. The recovery of DDVP was determined in the range from 105 % to 115 %, demonstrating the quantification accuracy of this method. The detection limit reached 4.7 μM, which was lower than the restricted amount in fruit set by the National Standard of China. The present method provides an efficient and user-friendly way for the detection of DDVP and many other organophosphorus pesticides in food.

Keywords: Alkaline phosphatase; Dichlorvos analysis; Fluorescent Tb-MOF.