A WRKY transcription factor, PyWRKY71, increased the activities of antioxidant enzymes and promoted the accumulation of cadmium in poplar

Abstract

Cadmium (Cd) pollution poses significant threats to the ecological environment and human health. Currently, phytoremediation is recognized as an environmentally friendly approach for mitigating Cd pollution, with increasing attention on the utilization of transgenic plants in Cd-contaminated soil remediation. In this study, we isolated and cloned PyWRKY71 from Populus yunnanensis and conducted a pot experiment to validate its enhanced functionality in conferring Cd tolerance to woody plants (poplar). During the experiment, the increase in plant height of the OE-87 line (overexpression poplar) was 1.46 times than that of the wild type (WT). Moreover, PyWRKY71 significantly promoted the accumulation of Cd in poplar, especially in the roots, where the Cd content in the OE-45 and OE-87 lines was 1.42 times than that in the WT. The chlorophyll content of transgenic poplar leaves was higher than that of the WT, reflecting a protective mechanism of PyWRKY71. Additionally, the activities of other antioxidants, including POD, SOD, CAT, and MDA, were elevated in transgenic poplars, bolstering their tolerance to Cd stress. In summary, PyWRKY71 exhibits substantial potential in regulating plant tolerance to Cd stress. This study not only provides a solid scientific foundation but also introduces a novel modified poplar variety for the remediation of Cd pollution.

Keywords: Antioxidant enzyme; Cadmium; Poplar; PyWRKY71.