Cu3-xP nanocrystals filled halloysite nanotubes for chemodynamic therapy of breast cancer

Puxiang Zhao; Jiaojiao Hu; Yue Feng; Feng Wu; Cuiying Tan; Xiaodan Chen; Mingxian Liu

doi:10.1016/j.jcis.2023.11.067

Cu_3-xP nanocrystals filled halloysite nanotubes for chemodynamic therapy of breast cancer

J Colloid Interface Sci. 2024 Feb:655:736-747. doi: 10.1016/j.jcis.2023.11.067. Epub 2023 Nov 11.

Authors

Puxiang Zhao¹, Jiaojiao Hu², Yue Feng¹, Feng Wu¹, Cuiying Tan¹, Xiaodan Chen³, Mingxian Liu⁴

Affiliations

¹ Department of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 511443, China.
² Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China.
³ Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications, College of Chemistry and Materials Science, Jinan University, Guangzhou 510632, China; International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China. Electronic address: chxdlzhsh@jnu.edu.cn.
⁴ Department of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 511443, China. Electronic address: liumx@jnu.edu.cn.

PMID: 37976747
DOI: 10.1016/j.jcis.2023.11.067

Abstract

Copper-based Fenton-like agents have the ability to convert weakly oxidizing H₂O₂ into highly oxidizing hydroxyl radicals (·OH) at tumor sites during chemodynamic therapy (CDT). In this study, the interfacial attraction properties between the negatively charged OCP^- in sodium phosphathynolate (NaOCP) and the positively charged environment inside the lumen of halloysite nanotubes (HNTs) were utilized to synthesize Cu_3-xP nanoparticles in situ within the HNTs. The study investigated the chemical composition, morphology, and structure of Cu_3-x P@HNTs. The results indicated uniform distribution of Cu_3-xP particles measuring 3-5 nm within HNTs' lumen. Experiments conducted internally and externally to cells confirmed the catalytic capability of Cu_3-xP@HNTs to oxidize H₂O₂ to ·OH. Furthermore, CP@H-CM was synthesized by enclosing Cu_3-xP@HNTs in a cancer cell membrane, which selectively targets cancer cells. The experiments revealed the cytotoxicity of CP@H-CM on 4T1 cells. Additionally, the antitumor efficacy of CP@H-CM was evaluated in vivo through tumor recurrence experiments in mice. Moreover, the efficacy of CP@H-CM in repressing tumor growth was enhanced by incorporating infrared laser, indicating a synergistic photodynamic treatment for breast cancer. This study presents an efficacious and viable therapeutic approach to inhibit postoperative tumor reappearance. The implications of this approach are promising, particularly in the domain of tumor treatment and metastasis.

Keywords: Chemodynamic therapy; Halloysite; Nanozyme; Selective modification.

MeSH terms

Animals
Clay / chemistry
Copper
Hydrogen Peroxide
Mice
Nanoparticles*
Nanotubes* / chemistry
Neoplasms*

Substances

Clay
Copper
Hydrogen Peroxide