Bovine papular stomatitis virus as a vaccine vector for cattle

Gustavo Delhon; Sushil Khatiwada; David Doub; Seth Harris; Sabal Chaulagain; Mostafa El-Gaffary; Daniel L Rock

doi:10.1099/jgv.0.001914

Bovine papular stomatitis virus as a vaccine vector for cattle

J Gen Virol. 2023 Nov;104(11):001914. doi: 10.1099/jgv.0.001914.

Authors

Gustavo Delhon¹, Sushil Khatiwada^{2

3}, David Doub², Seth Harris¹, Sabal Chaulagain^{2

4}, Mostafa El-Gaffary^{2

5}, Daniel L Rock²

Affiliations

¹ School of Veterinary Medicine & Biomedical Sciences, University of Nebraska, Lincoln, Nebraska, USA.
² Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, Illinois, USA.
³ Present address: Boehringer Ingelheim Animal Health, Ames, IA, USA.
⁴ Present address: Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA.
⁵ Present address: Department of Veterinary Clinical Pathology, College of Veterinary Medicine, Cairo University, Giza, Egypt.

Abstract

Virus vectored vaccines are not available commercially for cattle even though compelling potential applications exist. Bovine papular stomatitis virus (BPSV), a highly prevalent parapoxvirus, causes self-limited oral lesions in cattle. Ability of virus to accommodate large amounts of foreign DNA, induce low level of antiviral immunity, and circulate and likely persist in cattle populations, make BPSV an attractive candidate viral vector. Here, recombinant BPSV were constructed expressing either Bovine herpesvirus 1 (BoHV-1) glycoprotein gD (BPSV^gD), or gD and gB (BPSV^gD/gB). Immunization of BPSV serologically-positive calves with BPSV^gD or BPSV^gD/gB induced BoHV-1 neutralization antibodies and provided protection for three of four animals following a high dose BoHV-1 challenge at day 70 pi. Results indicate BPSV suitability as a candidate virus vector for cattle vaccines.

Keywords: bovine herpesvirus 1; bovine papular stomatitis virus; challenge; immunization; virus vector.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Antibodies, Viral
Cattle
Cattle Diseases* / prevention & control
Herpesvirus 1, Bovine* / genetics
Parapoxvirus* / genetics
Stomatitis*
Vaccines*
Viral Vaccines* / genetics

Substances

Vaccines
Antibodies, Viral
Viral Vaccines