The anti-inflammatory activity of probiotic Dadiah to activate Sirtuin-1 in inhibiting diabetic nephropathy progression

Rinita Amelia; Faridah Mohd Said; Farzana Yasmin; Harnavi Harun; Tofrizal Tofrizal

doi:10.1007/s40200-023-01265-7

The anti-inflammatory activity of probiotic Dadiah to activate Sirtuin-1 in inhibiting diabetic nephropathy progression

J Diabetes Metab Disord. 2023 Aug 9;22(2):1425-1442. doi: 10.1007/s40200-023-01265-7. eCollection 2023 Dec.

Authors

Rinita Amelia¹, Faridah Mohd Said², Farzana Yasmin², Harnavi Harun³, Tofrizal Tofrizal⁴

Affiliations

¹ Medical Faculty, Baiturrahmah University, Padang, West Sumatra Indonesia.
² Lincoln University College, Petaling Jaya, Selangor Malaysia.
³ Internist Medicine Department of Andalas University, Padang, West Sumatra Indonesia.
⁴ Pathology Anatomy Department of Medical Faculty Andalas University, Padang, West Sumatra Indonesia.

PMID: 37975108
PMCID: PMC10638242 (available on 2024-08-09)
DOI: 10.1007/s40200-023-01265-7

Abstract

Purpose: The activation of SIRT-1 in the kidney has become a new therapeutic target to increase resistance to many causal factors in DN development. Furthermore, antioxidative stress and anti-inflammation are essential to preventing renal fibrosis in DN. Therefore, finding "probiotic products" to treat and prevent DN is necessary. This study aimed to analyze the anti-inflammatory of probiotic dadiah to activate SIRT-1 in inhibiting DN progression.

Methods: This study is an experimental group designed with a post-test-only control group to observe the effect of dadiah, LAB, and bacteriocin on alloxan-induced nephropathy diabetic rats through two control groups and five intervention groups for eight weeks. The expression of antibodies SIRT-1 and TNF-α was examined using Immunohistochemistry and histopathology of kidney tissue. All data were analyzed using ANOVA test.

Results: The treatment of dadiah, lactic acid bacteria, and bacteriocin showed a higher expression of Sirtuin-1 than the positive control. They also, reduce TNF-α expression varies significantly between treatments. The highest average of interstitial fibrosis in the C + groups was substantially different from all groups, but all treatments showed decreased kidney fibrosis. Although all treatments showed a decrease in interstitial kidney fibrosis found in the control group, the treatment using dadiah showed the highest result.

Conclusions: Dadiah has the potential to the prevention of fibrosis on kidney tissue of alloxan-induced nephropathy diabetic rats. The findings could be to develop novel treatments for DN that aim to reduce the cascade of oxidative stress and inflammatory signals in kidney tissue.

Keywords: Dadiah; Diabetic Nephropathy; Sirtuin-1; TNF-α.

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