A phylogeny of the evening primrose family (Onagraceae) using a target enrichment approach with 303 nuclear loci

Rick P Overson; Matthew G Johnson; Lindsey L Bechen; Sylvia P Kinosian; Norman A Douglas; Jeremie B Fant; Peter C Hoch; Rachel A Levin; Michael J Moore; Robert A Raguso; Warren L Wagner; Krissa A Skogen; Norman J Wickett

doi:10.1186/s12862-023-02151-9

A phylogeny of the evening primrose family (Onagraceae) using a target enrichment approach with 303 nuclear loci

BMC Ecol Evol. 2023 Nov 17;23(1):66. doi: 10.1186/s12862-023-02151-9.

Authors

Rick P Overson¹, Matthew G Johnson², Lindsey L Bechen^{3

4}, Sylvia P Kinosian^{3

5}, Norman A Douglas⁶, Jeremie B Fant^{3

7}, Peter C Hoch⁸, Rachel A Levin⁹, Michael J Moore¹⁰, Robert A Raguso¹¹, Warren L Wagner¹², Krissa A Skogen^{3

7

13}, Norman J Wickett^{14

15

16}

Affiliations

¹ Arizona State University, PO Box 875502, Tempe, AZ, 85287, USA.
² Texas Tech University, Box 43131, Lubbock, TX, 79409, USA.
³ Negaunee Institute for Plant Conservation Science and Action, Chicago Botanic Garden, 1000 Lake Cook Rd, Glencoe, IL, 60022, USA.
⁴ Whitehead Institute for Biomedical Research, 455 Main St, Cambridge, MA, 02142, USA.
⁵ Department of Ecology and Evolutionary Biology, University of Arizona, PO Box 210088, Tucson, AZ, 85721, USA.
⁶ University of Florida, 220 Bartram Hall, Gainesville, FL, 32611, USA.
⁷ Northwestern University, 2205 Tech Dr, Evanston, IL, 60208, USA.
⁸ Missouri Botanical Garden, 4344 Shaw Blvd, St. Louis, MO, 63110, USA.
⁹ Amherst College, 25 East Dr, Amherst, MA, 01002, USA.
¹⁰ Oberlin College, 119 Woodland St, Oberlin, OH, 44074, USA.
¹¹ Cornell University, 215 Tower Rd, Ithaca, NY, 14853, USA.
¹² Smithsonian Institution, MRC-166, PO Box 37012, Washington, DC, 20013, USA.
¹³ Clemson University, 132 Long Hall, Clemson, SC, 29634, USA.
¹⁴ Negaunee Institute for Plant Conservation Science and Action, Chicago Botanic Garden, 1000 Lake Cook Rd, Glencoe, IL, 60022, USA. nwicket@clemson.edu.
¹⁵ Northwestern University, 2205 Tech Dr, Evanston, IL, 60208, USA. nwicket@clemson.edu.
¹⁶ Clemson University, 132 Long Hall, Clemson, SC, 29634, USA. nwicket@clemson.edu.

Abstract

Background: The evening primrose family (Onagraceae) includes 664 species (803 taxa) with a center of diversity in the Americas, especially western North America. Ongoing research in Onagraceae includes exploring striking variation in floral morphology, scent composition, and breeding system, as well as the role of these traits in driving diversity among plants and their interacting pollinators and herbivores. However, these efforts are limited by the lack of a comprehensive, well-resolved phylogeny. Previous phylogenetic studies based on a few loci strongly support the monophyly of the family and the sister relationship of the two largest tribes but fail to resolve several key relationships.

Results: We used a target enrichment approach to reconstruct the phylogeny of Onagraceae using 303 highly conserved, low-copy nuclear loci. We present a phylogeny for Onagraceae with 169 individuals representing 152 taxa sampled across the family, including extensive sampling within the largest tribe, Onagreae. Deep splits within the family are strongly supported, whereas relationships among closely related genera and species are characterized by extensive conflict among individual gene trees.

Conclusions: This phylogenetic resource will augment current research projects focused throughout the family in genomics, ecology, coevolutionary dynamics, biogeography, and the evolution of characters driving diversification in the family.

Keywords: Evening primrose; HybSeq; Onagraceae; Phylogenetics; Phylogenomics; Plant systematics; Target enrichment.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Genomics
Humans
Oenothera biennis* / genetics
Onagraceae*
Phylogeny
Plant Breeding

Abstract

Publication types

MeSH terms

Grants and funding