Predicting foodborne pathogens and probiotics taxa within poultry-related microbiomes using a machine learning approach

Moses B Ayoola; Nisha Pillai; Bindu Nanduri; Michael J Rothrock Jr; Mahalingam Ramkumar

doi:10.1186/s42523-023-00260-w

Predicting foodborne pathogens and probiotics taxa within poultry-related microbiomes using a machine learning approach

Anim Microbiome. 2023 Nov 15;5(1):57. doi: 10.1186/s42523-023-00260-w.

Authors

Moses B Ayoola^{1

2}, Nisha Pillai³, Bindu Nanduri², Michael J Rothrock Jr⁴, Mahalingam Ramkumar⁵

Affiliations

¹ Geosystems Research Institute, Mississippi State University, Starkville, MS, 39762, USA.
² Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, MS, 39762, USA.
³ Department of Computer Science and Engineering, Mississippi State University, Starkville, MS, 39762, USA.
⁴ Egg Safety and Quality Research Unit, USDA-ARS U.S. National Poultry Research Center, Athens, GA 30605, USA.
⁵ Department of Computer Science and Engineering, Mississippi State University, Starkville, MS, 39762, USA. ramkumar@cse.msstate.edu.

Abstract

Background: Microbiomes that can serve as an indicator of gut, intestinal, and general health of humans and animals are largely influenced by food consumed and contaminant bioagents. Microbiome studies usually focus on estimating the alpha (within sample) and beta (similarity/dissimilarity among samples) diversities. This study took a combinatorial approach and applied machine learning to microbiome data to predict the presence of disease-causing pathogens and their association with known/potential probiotic taxa. Probiotics are beneficial living microorganisms capable of improving the host organism's digestive system, immune function and ultimately overall health. Here, 16 S rRNA gene high-throughput Illumina sequencing of temporal pre-harvest (feces, soil) samples of 42 pastured poultry flocks (poultry in this entire work solely refers to chickens) from southeastern U.S. farms was used to generate the relative abundance of operational taxonomic units (OTUs) as machine learning input. Unique genera from the OTUs were used as predictors of the prevalence of foodborne pathogens (Salmonella, Campylobacter and Listeria) at different stages of poultry growth (START (2-4 weeks old), MID (5-7 weeks old), END (8-11 weeks old)), association with farm management practices and physicochemical properties.

Result: While we did not see any significant associations between known probiotics and Salmonella or Listeria, we observed significant negative correlations between known probiotics (Bacillus and Clostridium) and Campylobacter at the mid-time point of sample collection. Our data indicates a negative correlation between potential probiotics and Campylobacter at both early and end-time points of sample collection. Furthermore, our model prediction shows that changes in farm operations such as how often the houses are moved on the pasture, age at which chickens are introduced to the pasture, diet composition and presence of other animals on the farm could favorably increase the abundance and activity of probiotics that could reduce Campylobacter prevalence.

Conclusion: Integration of microbiome data with farm management practices using machine learning provided insights on how to reduce Campylobacter prevalence and transmission along the farm-to-fork continuum. Altering management practices to support proliferation of beneficial probiotics to reduce pathogen prevalence identified here could constitute a complementary method to the existing but ineffective interventions such as vaccination and bacteriophage cocktails usage. Study findings also corroborate the presence of bacterial genera such as Caloramator, DA101, Parabacteroides and Faecalibacterium as potential probiotics.

Keywords: Foodborne pathogens; Machine learning; Microbiome; Pastured poultry; Probiotics.