Visualizing calcium-dependent signaling networks in plants

Huimin Xu; Xinlin Liang; James R Lloyd; Yanmei Chen

doi:10.1016/j.tplants.2023.11.004

Visualizing calcium-dependent signaling networks in plants

Trends Plant Sci. 2024 Feb;29(2):117-119. doi: 10.1016/j.tplants.2023.11.004. Epub 2023 Nov 13.

Authors

Huimin Xu¹, Xinlin Liang², James R Lloyd³, Yanmei Chen⁴

Affiliations

¹ State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
² School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.
³ Department of Genetics, Institute for Plant Biotechnology, Stellenbosch University, 7600 Stellenbosch, South Africa.
⁴ State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: chenyanmei@cau.edu.cn.

PMID: 37968199
DOI: 10.1016/j.tplants.2023.11.004

Abstract

Calcium-dependent protein kinases (CDPKs) are a multigene protein kinase family that have key regulatory roles in plants. However, imaging CDPK signals in plant cells remains challenging. The recently developed genetically encoded CDPK-Förster resonance energy transfer (FRET) reporter developed by Liese et al. allows visualization of calcium (Ca²⁺)-dependent conformational changes during activation or inactivation of CDPKs, providing a powerful tool for real-time monitoring of calcium decoding in plants.

MeSH terms

Calcium* / metabolism
Plants* / genetics
Plants* / metabolism

Substances

Calcium