Integrating fecal pollution markers and fluorescence analysis for water quality assessment of urban river

Abstract

Human fecal contamination in urban rivers poses significant health risks, but their potential connections with other substances like dissolved organic matter (DOM) remain underexplored. In this study, five fecal pollution markers related to fecal Bacteroides or human fecal contamination (AllBac, HF183, BacH, Hum2, and Hum163) and DOM along an urban river were analyzed using quantitative polymerase chain reaction (qPCR) and three-dimensional excitation-emission (3D EEM) fluorescence spectrometry. All five markers were detected with average absolute abundance ranging from 2.51 to 6.28 lg gene copies/100 mL, showing a progressive increase along the river (R² = 0.29-0.92, p < 0.05). Parallel factor analysis identified three dominant DOM components (humic acid-like, fulvic acid-like, and protein-like), with strong positive correlations between protein-like components and all fecal markers (R² = 0.59-0.66, p < 0.001). Both fecal and DOM distributions consistently showed significant differences between upstream and downstream areas (p < 0.001), suggesting their complementary assessment. While DOM was more sensitive to environmental variables such as rainfall, rubber dam, and tidal dynamic, the combination of fecal pollution markers and 3D EEM analysis allowed a more comprehensive assessment of contamination levels, mitigating potential biases caused by the influence of multiple factors on a single method. Furthermore, due to the strong correlation between protein-like and fecal markers in the DOM, 3D EEM can be used as a pre-detection means for qPCR detection, reducing testing time and costs.

Keywords: Dissolved organic matter; Fecal pollution markers; Fluorescence spectroscopy; Human fecal contamination; Urban river.